Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 96022..96547 Replicon plasmid pWP5-S18-ESBL-08_1
Accession NZ_AP022162
Organism Escherichia coli strain WP5-S18-ESBL-08

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag H7R36_RS25420 Protein ID WP_001159868.1
Coordinates 96242..96547 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag H7R36_RS25415 Protein ID WP_000813634.1
Coordinates 96022..96240 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R36_RS25385 91415..91831 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
H7R36_RS25390 91828..92058 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H7R36_RS25395 92323..92823 + 501 WP_000528931.1 hypothetical protein -
H7R36_RS25400 92836..93609 + 774 WP_000905949.1 hypothetical protein -
H7R36_RS25405 93776..94909 + 1134 WP_001542067.1 DUF3800 domain-containing protein -
H7R36_RS25410 94943..95455 - 513 WP_000151784.1 hypothetical protein -
H7R36_RS25415 96022..96240 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R36_RS25420 96242..96547 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R36_RS25425 96548..97354 + 807 WP_000016982.1 site-specific integrase -
H7R36_RS25430 98128..98883 + 756 WP_024946706.1 replication initiation protein RepE -
H7R36_RS25435 99471..100637 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / tet(B) / sitABCD iutA / iucD / iucC / iucB / iucA 1..147186 147186


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T34779 WP_001159868.1 NZ_AP022162:96242-96547 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T34779 NZ_AP022162:96242-96547 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT34779 WP_000813634.1 NZ_AP022162:96022-96240 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT34779 NZ_AP022162:96022-96240 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References