Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 91415..92058 Replicon plasmid pWP5-S18-ESBL-08_1
Accession NZ_AP022162
Organism Escherichia coli strain WP5-S18-ESBL-08

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag H7R36_RS25385 Protein ID WP_001034046.1
Coordinates 91415..91831 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag H7R36_RS25390 Protein ID WP_001261278.1
Coordinates 91828..92058 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R36_RS25370 86552..86968 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
H7R36_RS25375 86965..87195 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H7R36_RS25380 87576..91370 + 3795 WP_001144732.1 hypothetical protein -
H7R36_RS25385 91415..91831 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
H7R36_RS25390 91828..92058 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7R36_RS25395 92323..92823 + 501 WP_000528931.1 hypothetical protein -
H7R36_RS25400 92836..93609 + 774 WP_000905949.1 hypothetical protein -
H7R36_RS25405 93776..94909 + 1134 WP_001542067.1 DUF3800 domain-containing protein -
H7R36_RS25410 94943..95455 - 513 WP_000151784.1 hypothetical protein -
H7R36_RS25415 96022..96240 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
H7R36_RS25420 96242..96547 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / tet(B) / sitABCD iutA / iucD / iucC / iucB / iucA 1..147186 147186


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T34778 WP_001034046.1 NZ_AP022162:c91831-91415 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T34778 NZ_AP022162:c91831-91415 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT34778 WP_001261278.1 NZ_AP022162:c92058-91828 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT34778 NZ_AP022162:c92058-91828 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References