Detailed information of TA system TA034776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 59069..59628
• Replicon: plasmid pWP5-S18-ESBL-08_1
• Accession: NZ_AP022162
• Organism: Escherichia coli strain WP5-S18-ESBL-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: H7R36_RS25230
• Protein ID: WP_000421272.1
• Coordinates: 59353..59628 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: H7R36_RS25225
• Protein ID: WP_001387467.1
• Coordinates: 59069..59353 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R36_RS25195	54532..54972	-	441	Protein_71	zinc-binding dehydrogenase	-
H7R36_RS25200	54946..55140	+	195	WP_000796505.1	hypothetical protein	-
H7R36_RS25205	55373..56263	+	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
H7R36_RS25210	56288..56668	+	381	WP_000710783.1	Rid family detoxifying hydrolase	-
H7R36_RS25215	56701..57666	+	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
H7R36_RS25220	57712..58464	-	753	WP_000562172.1	IclR family transcriptional regulator	-
H7R36_RS25225	59069..59353	+	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
H7R36_RS25230	59353..59628	+	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R36_RS25235	59734..60027	+	294	WP_001105060.1	hypothetical protein	-
H7R36_RS25240	60062..60190	-	129	Protein_80	IS481 family transposase	-
H7R36_RS25245	60223..61392	-	1170	WP_001072355.1	hypothetical protein	-
H7R36_RS25250	61885..62192	+	308	Protein_82	type II toxin-antitoxin system ParD family antitoxin	-
H7R36_RS25255	62535..62717	+	183	WP_042344623.1	hypothetical protein	-
H7R36_RS25260	62838..63578	+	741	WP_001066953.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / tet(B) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..147186	147186

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T34776 WP_000421272.1 NZ_AP022162:59353-59628 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T34776 NZ_AP022162:59353-59628 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT34776 WP_001387467.1 NZ_AP022162:59069-59353 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT34776 NZ_AP022162:59069-59353 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880