Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4364684..4365260 Replicon chromosome
Accession NZ_AP022161
Organism Escherichia coli strain WP5-S18-ESBL-08

Toxin (Protein)


Gene name shpA Uniprot ID A0A2X2XYK1
Locus tag H7R36_RS20895 Protein ID WP_001360091.1
Coordinates 4364973..4365260 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag H7R36_RS20890 Protein ID WP_000063156.1
Coordinates 4364684..4364986 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R36_RS20875 4361280..4363430 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
H7R36_RS20880 4363480..4363683 + 204 WP_000467859.1 YbdD/YjiX family protein -
H7R36_RS20885 4363694..4364650 + 957 WP_001297640.1 GTPase -
H7R36_RS20890 4364684..4364986 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
H7R36_RS20895 4364973..4365260 - 288 WP_001360091.1 BrnT family toxin Toxin
H7R36_RS20900 4365459..4366156 - 698 Protein_4095 IS1 family transposase -
H7R36_RS20905 4366213..4366308 + 96 Protein_4096 dienelactone hydrolase family protein -
H7R36_RS20910 4366454..4367686 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
H7R36_RS20915 4367727..4369007 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11325.80 Da        Isoelectric Point: 6.8821

>T34768 WP_001360091.1 NZ_AP022161:c4365260-4364973 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKTDRKERNRYEHG

Download         Length: 288 bp

>T34768 NZ_AP022161:c4365260-4364973 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAACAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT34768 WP_000063156.1 NZ_AP022161:c4364986-4364684 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT34768 NZ_AP022161:c4364986-4364684 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X2XYK1

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References