Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3739265..3739883 Replicon chromosome
Accession NZ_AP022159
Organism Escherichia coli strain WP5-S18-ESBL-07

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H7R21_RS17880 Protein ID WP_001291435.1
Coordinates 3739665..3739883 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H7R21_RS17875 Protein ID WP_000344800.1
Coordinates 3739265..3739639 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R21_RS17865 3734354..3735547 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H7R21_RS17870 3735570..3738719 + 3150 WP_182913337.1 efflux RND transporter permease AcrB -
H7R21_RS17875 3739265..3739639 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H7R21_RS17880 3739665..3739883 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
H7R21_RS17885 3740055..3740606 + 552 WP_060579167.1 maltose O-acetyltransferase -
H7R21_RS17890 3740722..3741192 + 471 WP_000136192.1 YlaC family protein -
H7R21_RS17895 3741356..3742906 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H7R21_RS17900 3742948..3743301 - 354 WP_001550741.1 DUF1428 family protein -
H7R21_RS17910 3743680..3743991 + 312 WP_000409911.1 MGMT family protein -
H7R21_RS17915 3744022..3744594 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T34735 WP_001291435.1 NZ_AP022159:3739665-3739883 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T34735 NZ_AP022159:3739665-3739883 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT34735 WP_000344800.1 NZ_AP022159:3739265-3739639 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT34735 NZ_AP022159:3739265-3739639 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References