Detailed information of TA system TA034732

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2605644..2606282
• Replicon: chromosome
• Accession: NZ_AP022159
• Organism: Escherichia coli strain WP5-S18-ESBL-07

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: H7R21_RS12385
• Protein ID: WP_000813795.1
• Coordinates: 2606106..2606282 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H7R21_RS12380
• Protein ID: WP_076797675.1
• Coordinates: 2605644..2606060 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R21_RS12360	2600796..2601737	-	942	WP_001251315.1	ABC transporter permease	-
H7R21_RS12365	2601738..2602751	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
H7R21_RS12370	2602769..2603914	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
H7R21_RS12375	2604159..2605565	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
H7R21_RS12380	2605644..2606060	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H7R21_RS12385	2606106..2606282	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H7R21_RS12390	2606504..2606734	+	231	WP_023910283.1	YncJ family protein	-
H7R21_RS12395	2606826..2608787	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H7R21_RS12400	2608860..2609396	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
H7R21_RS12405	2609449..2610660	+	1212	WP_071591517.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T34732 WP_000813795.1 NZ_AP022159:c2606282-2606106 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T34732 NZ_AP022159:c2606282-2606106 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT34732 WP_076797675.1 NZ_AP022159:c2606060-2605644 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT34732 NZ_AP022159:c2606060-2605644 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1