Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 86152..86795 Replicon plasmid pWP5-S18-ESBL-05_4
Accession NZ_AP022146
Organism Klebsiella quasipneumoniae strain WP5-S18-ESBL-05

Toxin (Protein)


Gene name vagD Uniprot ID A0A3Q8D6L7
Locus tag H7R80_RS29365 Protein ID WP_020804312.1
Coordinates 86152..86568 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag H7R80_RS29370 Protein ID WP_001261276.1
Coordinates 86565..86795 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R80_RS29340 81441..82409 + 969 WP_182939989.1 IS5 family transposase -
H7R80_RS29345 82467..83036 - 570 Protein_90 substrate-binding domain-containing protein -
H7R80_RS29350 83599..83799 - 201 WP_022631502.1 hypothetical protein -
H7R80_RS29355 83944..84912 - 969 WP_077254959.1 IS5 family transposase -
H7R80_RS29360 85233..86111 + 879 WP_009309916.1 restriction endonuclease -
H7R80_RS29365 86152..86568 - 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin Toxin
H7R80_RS29370 86565..86795 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7R80_RS29375 87377..87706 + 330 WP_048268852.1 hypothetical protein -
H7R80_RS29380 87747..88568 + 822 WP_048268865.1 hypothetical protein -
H7R80_RS29385 88565..89347 + 783 WP_048268853.1 site-specific integrase -
H7R80_RS29390 89770..90480 + 711 WP_048268854.1 hypothetical protein -
H7R80_RS29395 90552..91001 + 450 WP_117034938.1 hypothetical protein -
H7R80_RS29400 91144..91731 + 588 WP_182940175.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..122247 122247


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15154.59 Da        Isoelectric Point: 7.8637

>T34715 WP_020804312.1 NZ_AP022146:c86568-86152 [Klebsiella quasipneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T34715 NZ_AP022146:c86568-86152 [Klebsiella quasipneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT34715 WP_001261276.1 NZ_AP022146:c86795-86565 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT34715 NZ_AP022146:c86795-86565 [Klebsiella quasipneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q8D6L7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References