Detailed information of TA system TA034714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 49619..50262
• Replicon: plasmid pWP5-S18-ESBL-05_4
• Accession: NZ_AP022146
• Organism: Klebsiella quasipneumoniae strain WP5-S18-ESBL-05

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: H7R80_RS29165
• Protein ID: WP_182940178.1
• Coordinates: 49846..50262 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: H7R80_RS29160
• Protein ID: WP_001261282.1
• Coordinates: 49619..49849 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R80_RS29120	45145..45845	-	701	Protein_45	IS6-like element IS26 family transposase	-
H7R80_RS29125	45909..46376	-	468	Protein_46	tyrosine-type recombinase/integrase	-
H7R80_RS29130	46574..47590	-	1017	WP_017899884.1	hypothetical protein	-
H7R80_RS29135	47601..47915	-	315	WP_053389906.1	hypothetical protein	-
H7R80_RS29140	47942..48337	-	396	WP_017899885.1	hypothetical protein	-
H7R80_RS29145	48506..48811	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	-
H7R80_RS29150	48813..49031	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
H7R80_RS29155	49201..49662	-	462	WP_160866775.1	hypothetical protein	-
H7R80_RS29160	49619..49849	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H7R80_RS29165	49846..50262	+	417	WP_182940178.1	type II toxin-antitoxin system VapC family toxin	Toxin
H7R80_RS29170	50450..51154	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H7R80_RS29175	51294..51758	-	465	WP_001243598.1	isoprenylcysteine carboxylmethyltransferase family protein	-
H7R80_RS29180	52003..53661	-	1659	WP_000706606.1	FAD-dependent oxidoreductase	-
H7R80_RS29185	53823..54173	+	351	WP_000928911.1	metalloregulator ArsR/SmtB family transcription factor	-
H7R80_RS29190	54478..54954	-	477	WP_000043177.1	DNA starvation/stationary phase protection protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..122247	122247
	-	inside	IScluster/Tn	-	-	41515..51154	9639

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15215.63 Da
• Isoelectric Point: 6.4640

>T34714 WP_182940178.1 NZ_AP022146:49846-50262 [Klebsiella quasipneumoniae]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAVGAVLVTNNTREFERVPDLVLEDWVR

Nucleotide

• Length: 417 bp

>T34714 NZ_AP022146:49846-50262 [Klebsiella quasipneumoniae]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGTCGGTGCCGTGCTGGTGACGAATAATACGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT34714 WP_001261282.1 NZ_AP022146:49619-49849 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT34714 NZ_AP022146:49619-49849 [Klebsiella quasipneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGAAGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3