Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 48506..49031 Replicon plasmid pWP5-S18-ESBL-05_4
Accession NZ_AP022146
Organism Klebsiella quasipneumoniae strain WP5-S18-ESBL-05

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag H7R80_RS29145 Protein ID WP_013023785.1
Coordinates 48506..48811 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag H7R80_RS29150 Protein ID WP_001568025.1
Coordinates 48813..49031 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R80_RS29120 45145..45845 - 701 Protein_45 IS6-like element IS26 family transposase -
H7R80_RS29125 45909..46376 - 468 Protein_46 tyrosine-type recombinase/integrase -
H7R80_RS29130 46574..47590 - 1017 WP_017899884.1 hypothetical protein -
H7R80_RS29135 47601..47915 - 315 WP_053389906.1 hypothetical protein -
H7R80_RS29140 47942..48337 - 396 WP_017899885.1 hypothetical protein -
H7R80_RS29145 48506..48811 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R80_RS29150 48813..49031 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R80_RS29155 49201..49662 - 462 WP_160866775.1 hypothetical protein -
H7R80_RS29160 49619..49849 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H7R80_RS29165 49846..50262 + 417 WP_182940178.1 type II toxin-antitoxin system VapC family toxin -
H7R80_RS29170 50450..51154 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
H7R80_RS29175 51294..51758 - 465 WP_001243598.1 isoprenylcysteine carboxylmethyltransferase family protein -
H7R80_RS29180 52003..53661 - 1659 WP_000706606.1 FAD-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..122247 122247
- inside IScluster/Tn - - 41515..51154 9639


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T34713 WP_013023785.1 NZ_AP022146:c48811-48506 [Klebsiella quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T34713 NZ_AP022146:c48811-48506 [Klebsiella quasipneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT34713 WP_001568025.1 NZ_AP022146:c49031-48813 [Klebsiella quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT34713 NZ_AP022146:c49031-48813 [Klebsiella quasipneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References