Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 53467..55035 Replicon plasmid pWP5-S18-ESBL-05_3
Accession NZ_AP022145
Organism Klebsiella quasipneumoniae strain WP5-S18-ESBL-05

Toxin (Protein)


Gene name hipA Uniprot ID A0A859R327
Locus tag H7R80_RS28325 Protein ID WP_024134513.1
Coordinates 53728..55035 (+) Length 436 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A859R2S1
Locus tag H7R80_RS28320 Protein ID WP_039272366.1
Coordinates 53467..53718 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R80_RS28290 48967..49971 + 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
H7R80_RS28295 50250..50498 + 249 Protein_69 chemotaxis protein -
H7R80_RS28300 50689..50973 - 285 Protein_70 hypothetical protein -
H7R80_RS28305 51234..51515 + 282 WP_000575656.1 hypothetical protein -
H7R80_RS28310 51534..52097 + 564 WP_001132404.1 hypothetical protein -
H7R80_RS28315 52100..53104 + 1005 WP_001553091.1 tyrosine-type recombinase/integrase -
H7R80_RS28320 53467..53718 + 252 WP_039272366.1 helix-turn-helix domain-containing protein Antitoxin
H7R80_RS28325 53728..55035 + 1308 WP_024134513.1 type II toxin-antitoxin system HipA family toxin Toxin
H7R80_RS28330 55032..55526 + 495 WP_039272364.1 hypothetical protein -
H7R80_RS28335 55702..56640 - 939 WP_000395773.1 hypothetical protein -
H7R80_RS28340 56839..58479 + 1641 WP_088903423.1 PAAR domain-containing protein -
H7R80_RS28345 58851..59153 - 303 WP_001161555.1 hypothetical protein -
H7R80_RS28350 59373..59891 + 519 WP_079778878.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..161135 161135


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-64)


Sequences

Toxin        


Download         Length: 436 a.a.        Molecular weight: 47950.12 Da        Isoelectric Point: 6.2444

>T34712 WP_024134513.1 NZ_AP022145:53728-55035 [Klebsiella quasipneumoniae]
MQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQARFR
VETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTALL
WYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWSKS
GEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEPAS
SFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMFDMATKTEEVVA
TVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Download         Length: 1308 bp

>T34712 NZ_AP022145:53728-55035 [Klebsiella quasipneumoniae]
ATGCAGACGCTAACAGTGGCGATGAATGGTGAGGTCGTAGGAACACTGTACCGAGATAACAAAGGGGCCATGTCATTTCA
GTATGCCCCGGAGTGGATCTCGGAACCAGGTTCACGCGCCATTTCCATGTCACTGCCTTTGGGTCATGGCAGGATCACAG
GAAGTGAAGTCTTCAATTTCTTTAGCAACCTCCTACCCGACTCAGAGGCAATTATTGCCAGGATGCAGGCTAGGTTCAGA
GTTGAGACAACACATCCATTTGATTTGCTGGCGAGTGTTGGCCGGGATTGTGTTGGAGCTATCCAGCTCTATTCCCCCGA
TGCGGACATCCCAACTGTAATGGAGACGCTTGCAGAGCCTCTGGATGAAGCTCAGATTGAGCAACTGCTTGAAGGCTACC
AGGAAGCGCCCCTTGGGATGGCAGAGGATGCTGATTTTCGGATCTCGATTGCCGGAGCCCAAGAGAAAACTGCACTGCTT
TGGTATCAGGACCGCTGGCAACGCCCCCTGGGCAGCACCCCAACAAGCCATATTTTTAAGCTACCCATTGGTAAGATTGT
TCAAAATAACATCGATCTCAGTGAGAGTTGTGAGAATGAATGGCTCTGTTTGCGCATTGCAAAGGCCTTCGGTTTTGACG
TCGCCAATGCGGACCTAGCAACGTTCGGCAAGAAGAAGGTGCTTGTCGTTCAGCGTTTTGACCGGCGCTGGTCTAAATCC
GGCGAATGGCTTCTACGATTGCCCCAAGAAGACTTCTGCCAAGCTCTTGGGATCGCTCCTGCGCTCAAATACGAGTCACA
TGGAGGGCCAGGTATTGCTGACGCAATGAAGTTGTTGTTGGGGTCCAGACTTGCGGCTAAGGATAGAGAGCAATTTTTCA
AGTCGCAGATCCTATTCTGGATGTTGGCCGCGATTGATGGGCACGGGAAGAACTTCTCCATCTTCATTGAGCCTGCATCG
TCATTTAGGATGACCCCCCTCTACGATGTCATGTCTGCTTTTCCTATCTTCAAAAGCGGGGGAATTGCTGCTCAAAAGGC
AAAGATGGGGATGGCGCTCCAAGGCAAAAACCGCCAATACCATTTTGCGATGATCCAGCCTCGTCATTTTATCAGTACCG
CCGCCCATGTCGGGTTCCCACAAGATACCGCCGCTCAACTTATGTTCGACATGGCTACCAAGACAGAGGAGGTGGTTGCC
ACAGTAGCGGCTGAGCTGCCAGCCGGTTTCCCAGAGCACATCAGTAGCGCCATCTTCGAGGGGCTGTCCAGCCAGGCGGC
TAAGATCTTGAAGGGAGTAGCCAAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9369.79 Da        Isoelectric Point: 9.8930

>AT34712 WP_039272366.1 NZ_AP022145:53467-53718 [Klebsiella quasipneumoniae]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Download         Length: 252 bp

>AT34712 NZ_AP022145:53467-53718 [Klebsiella quasipneumoniae]
ATGAAAGTGACATCTCCATCCTTGCTTGCGGCTGCGGTGCGTGACCAGCGCAAGCTCAGCAAACTAACGCAGTCAGAGGC
GGCGAAACAGGTCGGAATCAAGCAGACGACCGTCTCTGATTTTGAACTACGGCCAGAATCTACCAAGCTGGAGACGCTAT
TCAAGCTCCTTTCTGCACTGGATCTGGAGCTTCATGTCGTGAAGCGGGGTTCAACATTGGACGATAGTAAAGTCTGGGAT
AGGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A859R327

Antitoxin

Source ID Structure
AlphaFold DB A0A859R2S1

References