Detailed information of TA system TA034710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 31941..32851
• Replicon: plasmid pWP5-S18-ESBL-05_2
• Accession: NZ_AP022144
• Organism: Klebsiella quasipneumoniae strain WP5-S18-ESBL-05

Toxin (Protein)

• Gene name: -
• Uniprot ID: R4YBC5
• Locus tag: H7R80_RS27200
• Protein ID: WP_004144067.1
• Coordinates: 32381..32851 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6A8PHD0
• Locus tag: H7R80_RS27195
• Protein ID: WP_023287184.1
• Coordinates: 31941..32384 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R80_RS27175	27834..28745	+	912	Protein_38	ThiF family adenylyltransferase	-
H7R80_RS27180	28814..30034	+	1221	WP_000343760.1	ISL3-like element ISKox3 family transposase	-
H7R80_RS27185	30064..30645	+	582	Protein_40	Mov34/MPN/PAD-1 family protein	-
H7R80_RS27190	30692..31840	+	1149	Protein_41	translesion error-prone DNA polymerase V subunit UmuC	-
H7R80_RS27195	31941..32384	+	444	WP_023287184.1	DUF2384 domain-containing protein	Antitoxin
H7R80_RS27200	32381..32851	+	471	WP_004144067.1	RES family NAD+ phosphorylase	Toxin
H7R80_RS27205	32938..33933	+	996	WP_077254794.1	IS5 family transposase	-
H7R80_RS27210	33935..34903	+	969	WP_072096747.1	IS5 family transposase	-
H7R80_RS27215	35092..35352	-	261	WP_017901409.1	hypothetical protein	-
H7R80_RS27220	35657..36625	+	969	WP_074423483.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..182573	182573
	-	inside	IScluster/Tn	-	-	23889..36625	12736

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17573.83 Da
• Isoelectric Point: 4.5152

>T34710 WP_004144067.1 NZ_AP022144:32381-32851 [Klebsiella quasipneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T34710 NZ_AP022144:32381-32851 [Klebsiella quasipneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16522.84 Da
• Isoelectric Point: 10.3013

>AT34710 WP_023287184.1 NZ_AP022144:31941-32384 [Klebsiella quasipneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT34710 NZ_AP022144:31941-32384 [Klebsiella quasipneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAAGTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACTGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A384IED8

Antitoxin

• AlphaFold DB: A0A6A8PHD0