Detailed information of TA system TA034709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 115575..116097
• Replicon: plasmid pWP5-S18-ESBL-05_1
• Accession: NZ_AP022143
• Organism: Klebsiella quasipneumoniae strain WP5-S18-ESBL-05

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A7H0EVH6
• Locus tag: H7R80_RS26460
• Protein ID: WP_014839901.1
• Coordinates: 115575..115859 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A7H0EVH5
• Locus tag: H7R80_RS26465
• Protein ID: WP_014839900.1
• Coordinates: 115849..116097 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R80_RS26425	110831..111040	+	210	WP_125316032.1	hypothetical protein	-
H7R80_RS26430	111103..112047	+	945	WP_014839903.1	DsbA family protein	-
H7R80_RS26435	112128..112649	+	522	WP_048336588.1	hypothetical protein	-
H7R80_RS26440	112646..113248	+	603	WP_014839902.1	hypothetical protein	-
H7R80_RS26445	113489..114208	+	720	WP_182940093.1	cation:proton antiporter	-
H7R80_RS26450	114225..114902	-	678	WP_182940094.1	hypothetical protein	-
H7R80_RS26455	114908..115384	-	477	WP_042934526.1	hypothetical protein	-
H7R80_RS26460	115575..115859	-	285	WP_014839901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R80_RS26465	115849..116097	-	249	WP_014839900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H7R80_RS26470	116438..117841	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
H7R80_RS26475	117870..118502	-	633	WP_001567369.1	hypothetical protein	-
H7R80_RS26480	118682..118966	-	285	WP_182940095.1	hypothetical protein	-
H7R80_RS26485	119207..119500	-	294	WP_154923539.1	hypothetical protein	-
H7R80_RS26490	119554..120162	-	609	WP_182940096.1	DUF2913 family protein	-
H7R80_RS26495	120299..120697	-	399	WP_014839899.1	H-NS histone family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-62 / aadA2 / qacE / sul1	-	1..215703	215703
	-	flank	IS/Tn	-	-	116438..117841	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10866.46 Da
• Isoelectric Point: 10.5831

>T34709 WP_014839901.1 NZ_AP022143:c115859-115575 [Klebsiella quasipneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDIYNATRHRN

Nucleotide

• Length: 285 bp

>T34709 NZ_AP022143:c115859-115575 [Klebsiella quasipneumoniae]
ATGACCTATAAGTTAGCCTTCAATGAATCAGCTTTGAAAGAATGGAAAAAACTGGGTCATACTATTCGGGAGCAATTCAA
AAAGAAGCTTTCCGAACGGCTTCAGAACCCACGGGTGCCCGCAGCACAACTCCATGGAGGTAAAGACCAGTATAAAATAA
AGCTCCATGGCGCGGGTTACCGACTGGTTTACAGTGTCAACGATGAAATTGTGACGGTCACCGTAATTGGGGTGGGCAAA
CGCAACAATGATGATATCTACAACGCAACCCGACACAGAAATTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9071.29 Da
• Isoelectric Point: 3.8832

>AT34709 WP_014839900.1 NZ_AP022143:c116097-115849 [Klebsiella quasipneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL

Nucleotide

• Length: 249 bp

>AT34709 NZ_AP022143:c116097-115849 [Klebsiella quasipneumoniae]
ATGGCATTTCAGGTTTTAACCACCACAGCAGCCAGCATTACAGAACTTAAACGTGACCCTATGGGTACCTTCAATGCAGG
AGAAGGTGCACCGGTAGCAATCCTGAATCGCAACGAACCCGCTTTTTACTGTGTACCACCAGCCCTGTATGCTCAGCTGA
TGGAAATGCTGGAAGATCAAGAGTTGGGGCGTATTGCTGATGAACGAATCGGTGAGGACCGAATCGAGGTCAACATTGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: A0A7H0EVH6

Antitoxin

• AlphaFold DB: A0A7H0EVH5