Detailed information of TA system TA034708

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 77612..78207
• Replicon: plasmid pWP5-S18-ESBL-05_1
• Accession: NZ_AP022143
• Organism: Klebsiella quasipneumoniae strain WP5-S18-ESBL-05

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: H7R80_RS26285
• Protein ID: WP_182940150.1
• Coordinates: 77830..78207 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: H7R80_RS26280
• Protein ID: WP_182940149.1
• Coordinates: 77612..77833 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R80_RS26255	73901..74323	+	423	WP_182940162.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
H7R80_RS26260	74323..75594	+	1272	WP_182940145.1	translesion error-prone DNA polymerase V subunit UmuC	-
H7R80_RS26265	75688..75975	+	288	WP_182940146.1	DUF1778 domain-containing protein	-
H7R80_RS26270	75963..76448	+	486	WP_182940147.1	GNAT family N-acetyltransferase	-
H7R80_RS26275	76539..76799	-	261	WP_182940148.1	hypothetical protein	-
H7R80_RS26280	77612..77833	+	222	WP_182940149.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H7R80_RS26285	77830..78207	+	378	WP_182940150.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
H7R80_RS26290	78682..78942	+	261	WP_182940151.1	hypothetical protein	-
H7R80_RS26295	79021..79992	+	972	WP_182940152.1	Hok/Gef family protein	-
H7R80_RS26300	80184..80561	-	378	WP_182940153.1	phosphonate ABC transporter substrate-binding protein	-
H7R80_RS26305	82378..82665	+	288	WP_182940154.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-62 / aadA2 / qacE / sul1	-	1..215703	215703
	-	flank	IS/Tn	-	-	72575..73795	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-52)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13795.95 Da
• Isoelectric Point: 6.2182

>T34708 WP_182940150.1 NZ_AP022143:77830-78207 [Klebsiella quasipneumoniae]
MKWVSAKEVIGIHDRLLALLAGVPGMPDPDRAEAIIYRVQNRHFYESVDDLFELAATYLVAIARGHIFNDGNKRTALAVS
MLFLRRNGIVLAVTGSVLDDLTVDAATGDLLVDQVAQVLRKQCYN

Nucleotide

• Length: 378 bp

>T34708 NZ_AP022143:77830-78207 [Klebsiella quasipneumoniae]
ATGAAGTGGGTTAGCGCTAAAGAGGTTATCGGCATCCACGACCGCCTGTTAGCGTTGCTGGCGGGGGTGCCGGGAATGCC
AGATCCGGACCGTGCTGAAGCCATTATATACCGGGTTCAAAATCGACATTTCTATGAATCAGTTGATGATCTCTTTGAGC
TGGCTGCAACTTATCTGGTGGCTATCGCTCGTGGGCATATCTTCAACGACGGGAATAAACGTACAGCTCTCGCGGTCTCG
ATGTTGTTTCTTCGACGCAATGGGATCGTTCTGGCTGTTACCGGGTCAGTACTGGACGATTTGACGGTTGACGCGGCCAC
AGGCGATTTGCTGGTGGACCAGGTGGCCCAAGTGCTACGTAAGCAATGCTACAACTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8278.16 Da
• Isoelectric Point: 4.4000

>AT34708 WP_182940149.1 NZ_AP022143:77612-77833 [Klebsiella quasipneumoniae]
MKSYTATEARQSISEVMDIASSGEPVEITRRDGTSNVLISREEFDAWQNQKLDAEFADIMQHYGKPIEALTNR

Nucleotide

• Length: 222 bp

>AT34708 NZ_AP022143:77612-77833 [Klebsiella quasipneumoniae]
ATGAAATCATATACTGCAACCGAAGCCCGGCAATCGATCTCCGAAGTGATGGACATCGCCTCCTCCGGGGAACCTGTGGA
GATCACCCGTCGTGATGGAACGTCAAATGTCCTGATTAGCCGGGAAGAGTTCGACGCCTGGCAAAATCAGAAGCTGGATG
CCGAATTTGCGGACATCATGCAGCATTATGGCAAACCAATTGAAGCGTTGACCAACCGATGA