Detailed information of TA system TA034675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1818344..1818934
• Replicon: chromosome
• Accession: NZ_AP022139
• Organism: Klebsiella pneumoniae strain WP5-S18-ESBL-02

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: H7R18_RS08775
• Protein ID: WP_023341911.1
• Coordinates: 1818602..1818934 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: H7R18_RS08770
• Protein ID: WP_000288812.1
• Coordinates: 1818344..1818601 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R18_RS08750	1814169..1814411	-	243	WP_040219557.1	hypothetical protein	-
H7R18_RS08755	1814678..1816426	+	1749	WP_094638131.1	hypothetical protein	-
H7R18_RS08760	1817346..1817806	+	461	Protein_1715	hypothetical protein	-
H7R18_RS08765	1817803..1818009	+	207	WP_020805021.1	helix-turn-helix domain-containing protein	-
H7R18_RS08770	1818344..1818601	+	258	WP_000288812.1	antitoxin	Antitoxin
H7R18_RS08775	1818602..1818934	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H7R18_RS08780	1819780..1821453	+	1674	WP_000940059.1	type I restriction-modification system subunit M	-
H7R18_RS08785	1821450..1823015	+	1566	WP_001081246.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T34675 WP_023341911.1 NZ_AP022139:1818602-1818934 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T34675 NZ_AP022139:1818602-1818934 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT34675 WP_000288812.1 NZ_AP022139:1818344-1818601 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT34675 NZ_AP022139:1818344-1818601 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0