Detailed information of TA system TA034671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 173902..174803
• Replicon: plasmid pWP5-S18-CRE-02_1
• Accession: NZ_AP022127
• Organism: Enterobacter cloacae strain WP5-S18-CRE-02

Toxin (Protein)

• Gene name: -
• Uniprot ID: S1EZ83
• Locus tag: H7R61_RS26140
• Protein ID: WP_008786589.1
• Coordinates: 173902..174360 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: H7R61_RS26145
• Protein ID: WP_008786588.1
• Coordinates: 174357..174803 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R61_RS26095	169628..169870	+	243	WP_007372291.1	hypothetical protein	-
H7R61_RS26100	169881..170279	+	399	WP_007372292.1	hypothetical protein	-
H7R61_RS26105	170363..170689	+	327	WP_007372293.1	hypothetical protein	-
H7R61_RS26110	171052..171390	-	339	WP_007372294.1	hypothetical protein	-
H7R61_RS26115	171628..172071	+	444	WP_007372295.1	hypothetical protein	-
H7R61_RS26120	172190..172531	+	342	WP_007372296.1	hypothetical protein	-
H7R61_RS26125	172690..173244	+	555	WP_007372297.1	hypothetical protein	-
H7R61_RS26130	173294..173560	+	267	WP_007372298.1	hypothetical protein	-
H7R61_RS26135	173628..173858	-	231	WP_007372299.1	hypothetical protein	-
H7R61_RS26140	173902..174360	-	459	WP_008786589.1	RES family NAD+ phosphorylase	Toxin
H7R61_RS26145	174357..174803	-	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
H7R61_RS26150	174952..175269	+	318	WP_016241555.1	hypothetical protein	-
H7R61_RS26155	175311..176153	+	843	WP_007372303.1	hypothetical protein	-
H7R61_RS26160	176125..176403	+	279	WP_007372304.1	hypothetical protein	-
H7R61_RS26165	176463..176906	+	444	WP_007372305.1	hypothetical protein	-
H7R61_RS26170	177193..177357	+	165	WP_007372306.1	hypothetical protein	-
H7R61_RS26175	177925..178767	+	843	WP_007372307.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..207601	207601

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17005.43 Da
• Isoelectric Point: 4.5730

>T34671 WP_008786589.1 NZ_AP022127:c174360-173902 [Enterobacter cloacae]
VILYRLTKTRYLMTAWSGLGAKETGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T34671 NZ_AP022127:c174360-173902 [Enterobacter cloacae]
GTGATCCTTTACCGACTGACCAAGACCCGTTATCTGATGACTGCGTGGTCTGGTCTGGGTGCGAAAGAAACGGGGGGCCG
CTGGAACAGCGTAGGCACTGCAATGGTCTACCTGTCTGAAACGGCCAGCCTTACCATGCTTGAAACGCTGGTACACATTC
ACGCGCCGCAGTTACTGGACGACTTTACACTGCTGAGTCTTGATGTACCGGATGATCAGATCCAGACCTTCGACATGAGC
CGTCTCCCTGATAACTGGGCGAGTGAGGATGCTCCTGCTGAGCTGGCGCTCTACGGTGATAACTGGGCTGAAAGCGGTTC
TTCTATCGCGCTGCGTATTCCAAGTGCGTTGTCTCCGGTTGAATTTAACTACTTGCTGAATCCGGCACACCCTGAACTCT
TTGACCTGATGGCTACGGTTAAGAAAATTCCGTTCCGGTTTGATTCCCGCCTGAAATAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT34671 WP_008786588.1 NZ_AP022127:c174803-174357 [Enterobacter cloacae]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT34671 NZ_AP022127:c174803-174357 [Enterobacter cloacae]
ATGACGATGAAAGTTTTTTCTCCAGACGTTACCAAAACGAATCAGCACTGCTTATGGCAGGTTGCTGGGCTGGATAATGA
CGGTATCGCGCTGATGGATAAAATCAATCACGGTCTCGATGGCACAGTAGCTAAGCACATTTCTGAATGGGCTAACATCA
CGCCGTCTGAGCTGCGCAAAATGTCGGGTATACCTAATACGACGTTTAATCGTAGCATCAAAGACCGTTTCACTGCCGAT
CAGAGCGAACGTCTGGTGCGCATTATCCGCGTAATTGAACGCGCTGTAGAGCTGTTTGAAGGTGACAAAGAAGCGGCGCA
CAAATGGCTGAACGAAGCGAACCGTGGTCTGAGCTGGAAATCACCGGCAGAGCTTGTTTCTTCTGAGACAGGTGCGCTTG
AAGTGATGCGCCTGATTACGCGTATTGAGCACGGGGTATACTCGTGA

Structures

Toxin

• AlphaFold DB: S1EZ83