Detailed information of TA system TA034670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 166603..167153
• Replicon: plasmid pWP5-S18-CRE-02_1
• Accession: NZ_AP022127
• Organism: Enterobacter cloacae strain WP5-S18-CRE-02

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: H7R61_RS26070
• Protein ID: WP_007372286.1
• Coordinates: 166845..167153 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: H7R61_RS26065
• Protein ID: WP_007372285.1
• Coordinates: 166603..166842 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R61_RS26030	162385..163089	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H7R61_RS26035	163227..163424	+	198	WP_032155220.1	Lar family restriction alleviation protein	-
H7R61_RS26040	163477..163935	+	459	WP_008786599.1	hypothetical protein	-
H7R61_RS26045	164083..164223	+	141	WP_007372281.1	hypothetical protein	-
H7R61_RS26050	164239..164871	+	633	WP_007372282.1	hypothetical protein	-
H7R61_RS26055	165332..166003	+	672	WP_007372283.1	hypothetical protein	-
H7R61_RS26060	166000..166464	+	465	WP_008786596.1	hypothetical protein	-
H7R61_RS26065	166603..166842	+	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H7R61_RS26070	166845..167153	+	309	WP_007372286.1	CcdB family protein	Toxin
H7R61_RS26075	167174..167332	+	159	WP_182945090.1	hypothetical protein	-
H7R61_RS26080	167487..168041	+	555	WP_007372288.1	hypothetical protein	-
H7R61_RS26085	168111..168728	+	618	WP_007372289.1	hypothetical protein	-
H7R61_RS26090	168811..169281	+	471	WP_007372290.1	hypothetical protein	-
H7R61_RS26095	169628..169870	+	243	WP_007372291.1	hypothetical protein	-
H7R61_RS26100	169881..170279	+	399	WP_007372292.1	hypothetical protein	-
H7R61_RS26105	170363..170689	+	327	WP_007372293.1	hypothetical protein	-
H7R61_RS26110	171052..171390	-	339	WP_007372294.1	hypothetical protein	-
H7R61_RS26115	171628..172071	+	444	WP_007372295.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..207601	207601

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11351.17 Da
• Isoelectric Point: 8.5044

>T34670 WP_007372286.1 NZ_AP022127:166845-167153 [Enterobacter cloacae]
MQYTVYRNPGNSQAYPYLLDIQSDIIGELNTRLVIPLHRLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKSAVDFLLDGF

Nucleotide

• Length: 309 bp

>T34670 NZ_AP022127:166845-167153 [Enterobacter cloacae]
ATGCAATATACCGTTTACCGCAATCCCGGCAACAGCCAGGCATACCCTTACCTGCTGGATATACAGAGCGACATCATTGG
CGAGTTAAATACCCGCCTGGTGATCCCCCTTCATCGGCTTAAAAAAGGAGCCTCGGCCCCGGTTGCGCGATTAACTCCGG
TCATACAGGTGGAAGGGAATGACGTTATCCTGATGACGCATGAAATGGCTTCAGTCCGCGTGAAGCAGCTCGGTCAGGCG
GTCATGGACGCATCTCCGTTCCGGCACACTATCAAAAGTGCGGTGGACTTCCTGCTGGATGGTTTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8784.97 Da
• Isoelectric Point: 8.6387

>AT34670 WP_007372285.1 NZ_AP022127:166603-166842 [Enterobacter cloacae]
MQATAARQKKTVSVTLEPMLVQQAKDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Nucleotide

• Length: 240 bp

>AT34670 NZ_AP022127:166603-166842 [Enterobacter cloacae]
ATGCAGGCTACAGCTGCTCGCCAGAAAAAAACCGTCAGTGTCACCCTTGAGCCAATGCTCGTACAGCAGGCAAAAGACGC
TGGGATTAACTTGTCCGCCACGCTGGCAGAAGCGCTTCAGAGCAAACTGAAGGCCAGTGCAGCGGAAGAATGGAAACGAC
AGAATCGTGCTGGTATTCAGGAGTTGAACCGCATCACTGAAGAGCATGGGTTACTGTCTGACGAATACAGGACGTTCTGA

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3