Detailed information of TA system TA034650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 17842..18401
• Replicon: plasmid pWP5-W18-ESBL-11_2
• Accession: NZ_AP022122
• Organism: Escherichia coli strain WP5-W18-ESBL-11

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: H7R04_RS23625
• Protein ID: WP_000421259.1
• Coordinates: 18126..18401 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: H7R04_RS23620
• Protein ID: WP_001178087.1
• Coordinates: 17842..18126 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R04_RS23600	13491..14024	-	534	WP_001372168.1	transcription termination factor NusG	-
H7R04_RS23605	14466..14753	-	288	WP_000074849.1	conjugal transfer protein TraA	-
H7R04_RS23610	14671..14934	-	264	WP_001324596.1	ash family protein	-
H7R04_RS23615	15907..16938	+	1032	WP_000907852.1	replication initiation protein	-
H7R04_RS23620	17842..18126	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
H7R04_RS23625	18126..18401	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R04_RS23630	18507..18788	+	282	WP_001105066.1	hypothetical protein	-
H7R04_RS23635	18980..19246	-	267	WP_000627832.1	hypothetical protein	-
H7R04_RS23640	19246..19740	-	495	WP_015058884.1	hypothetical protein	-
H7R04_RS23645	19796..20398	-	603	WP_061671620.1	proQ/FINO family protein	-
H7R04_RS23650	20752..22098	+	1347	WP_001132027.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-8	-	1..89476	89476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T34650 WP_000421259.1 NZ_AP022122:18126-18401 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T34650 NZ_AP022122:18126-18401 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT34650 WP_001178087.1 NZ_AP022122:17842-18126 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT34650 NZ_AP022122:17842-18126 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625