Detailed information of TA system TA034649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 123886..124262
• Replicon: plasmid pWP5-W18-ESBL-11_1
• Accession: NZ_AP022121
• Organism: Escherichia coli strain WP5-W18-ESBL-11

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: H7R04_RS23405
• Protein ID: WP_000969988.1
• Coordinates: 124080..124262 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: H7R04_RS23400
• Protein ID: WP_001324224.1
• Coordinates: 123886..124083 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R04_RS23380	119146..119382	+	237	WP_001323890.1	colicin V immunity protein	-
H7R04_RS23385	119360..119671	+	312	WP_001259758.1	colicin V	-
H7R04_RS23390	119841..121955	-	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
H7R04_RS23395	121930..123204	-	1275	WP_000489609.1	HlyD family secretion protein	-
H7R04_RS23400	123886..124083	+	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
H7R04_RS23405	124080..124262	+	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R04_RS23410	124361..124669	+	309	WP_000377483.1	hypothetical protein	-
H7R04_RS23415	125229..126263	+	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / blaTEM-1B	iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB	1..158291	158291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T34649 WP_000969988.1 NZ_AP022121:124080-124262 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T34649 NZ_AP022121:124080-124262 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT34649 WP_001324224.1 NZ_AP022121:123886-124083 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT34649 NZ_AP022121:123886-124083 [Escherichia coli]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A075MDK3