Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 82805..83430 Replicon plasmid pWP5-W18-ESBL-11_1
Accession NZ_AP022121
Organism Escherichia coli strain WP5-W18-ESBL-11

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag H7R04_RS23210 Protein ID WP_000911313.1
Coordinates 82805..83203 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID V0VCB2
Locus tag H7R04_RS23215 Protein ID WP_000450520.1
Coordinates 83203..83430 (-) Length 76 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R04_RS23195 79130..79627 + 498 WP_000605857.1 entry exclusion protein -
H7R04_RS23200 79659..80390 + 732 WP_023565183.1 complement resistance protein TraT -
H7R04_RS23205 80643..82796 + 2154 WP_000009324.1 type IV conjugative transfer system coupling protein TraD -
H7R04_RS23210 82805..83203 - 399 WP_000911313.1 tRNA(fMet)-specific endonuclease VapC Toxin
H7R04_RS23215 83203..83430 - 228 WP_000450520.1 toxin-antitoxin system antitoxin VapB Antitoxin

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / blaTEM-1B iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB 1..158291 158291


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-125)

Antitoxin

(8-50)


Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 14901.15 Da        Isoelectric Point: 7.8605

>T34648 WP_000911313.1 NZ_AP022121:c83203-82805 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Download         Length: 399 bp

>T34648 NZ_AP022121:c83203-82805 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8596.63 Da        Isoelectric Point: 4.7513

>AT34648 WP_000450520.1 NZ_AP022121:c83430-83203 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT34648 NZ_AP022121:c83430-83203 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB V0VCB2

References