Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 32827..33470 Replicon plasmid pWP5-W18-ESBL-11_1
Accession NZ_AP022121
Organism Escherichia coli strain WP5-W18-ESBL-11

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag H7R04_RS22885 Protein ID WP_001034046.1
Coordinates 32827..33243 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag H7R04_RS22890 Protein ID WP_001261278.1
Coordinates 33240..33470 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R04_RS22870 27964..28380 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
H7R04_RS22875 28377..28607 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H7R04_RS22880 28988..32782 + 3795 WP_001144731.1 hypothetical protein -
H7R04_RS22885 32827..33243 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
H7R04_RS22890 33240..33470 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7R04_RS22895 33735..34235 + 501 WP_000528932.1 hypothetical protein -
H7R04_RS22900 34248..35021 + 774 WP_000905949.1 hypothetical protein -
H7R04_RS22905 35188..36321 + 1134 WP_000545984.1 DUF3800 domain-containing protein -
H7R04_RS22910 36355..36867 - 513 WP_000151784.1 hypothetical protein -
H7R04_RS22915 37435..37653 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
H7R04_RS22920 37655..37960 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / blaTEM-1B iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB 1..158291 158291


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T34642 WP_001034046.1 NZ_AP022121:c33243-32827 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T34642 NZ_AP022121:c33243-32827 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT34642 WP_001261278.1 NZ_AP022121:c33470-33240 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT34642 NZ_AP022121:c33470-33240 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References