Detailed information of TA system TA034635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3518415..3519252
• Replicon: chromosome
• Accession: NZ_AP022120
• Organism: Escherichia coli strain WP5-W18-ESBL-11

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: H7R04_RS16860
• Protein ID: WP_000227784.1
• Coordinates: 3518710..3519252 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: H7R04_RS16855
• Protein ID: WP_001297137.1
• Coordinates: 3518415..3518726 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R04_RS16830	3513435..3514382	+	948	WP_021548571.1	cytochrome o ubiquinol oxidase subunit II	-
H7R04_RS16835	3514404..3516395	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
H7R04_RS16840	3516385..3516999	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
H7R04_RS16845	3516999..3517328	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
H7R04_RS16850	3517340..3518230	+	891	WP_000971336.1	heme o synthase	-
H7R04_RS16855	3518415..3518726	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
H7R04_RS16860	3518710..3519252	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
H7R04_RS16865	3519308..3520243	-	936	WP_001297127.1	sel1 repeat family protein	-
H7R04_RS16870	3520651..3522015	+	1365	WP_001000978.1	MFS transporter	-
H7R04_RS16875	3522143..3522634	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
H7R04_RS16880	3522802..3523713	+	912	WP_000705847.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T34635 WP_000227784.1 NZ_AP022120:3518710-3519252 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T34635 NZ_AP022120:3518710-3519252 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT34635 WP_001297137.1 NZ_AP022120:3518415-3518726 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT34635 NZ_AP022120:3518415-3518726 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3