Detailed information of TA system TA034632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2446036..2446674
• Replicon: chromosome
• Accession: NZ_AP022120
• Organism: Escherichia coli strain WP5-W18-ESBL-11

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: H7R04_RS11635
• Protein ID: WP_000813794.1
• Coordinates: 2446498..2446674 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H7R04_RS11630
• Protein ID: WP_001270286.1
• Coordinates: 2446036..2446452 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R04_RS11605	2441353..2442006	-	654	Protein_2271	ABC transporter ATP-binding protein	-
H7R04_RS11610	2442022..2442719	-	698	Protein_2272	IS1 family transposase	-
H7R04_RS11615	2442775..2443143	-	369	Protein_2273	ABC transporter ATP-binding protein	-
H7R04_RS11620	2443161..2444306	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
H7R04_RS11625	2444551..2445957	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
H7R04_RS11630	2446036..2446452	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H7R04_RS11635	2446498..2446674	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H7R04_RS11640	2446896..2447126	+	231	WP_000494244.1	YncJ family protein	-
H7R04_RS11645	2447218..2449179	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H7R04_RS11650	2449252..2449788	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
H7R04_RS11655	2449841..2451055	+	1215	WP_001349372.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2451095..2452360	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T34632 WP_000813794.1 NZ_AP022120:c2446674-2446498 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T34632 NZ_AP022120:c2446674-2446498 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT34632 WP_001270286.1 NZ_AP022120:c2446452-2446036 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT34632 NZ_AP022120:c2446452-2446036 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB