Detailed information of TA system TA034620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1359110..1359735
• Replicon: chromosome
• Accession: NZ_AP022120
• Organism: Escherichia coli strain WP5-W18-ESBL-11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H7R04_RS06575
• Protein ID: WP_000911330.1
• Coordinates: 1359337..1359735 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: H7R04_RS06570
• Protein ID: WP_000450524.1
• Coordinates: 1359110..1359337 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R04_RS06545	1354913..1355383	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
H7R04_RS06550	1355383..1355955	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
H7R04_RS06555	1356101..1356979	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
H7R04_RS06560	1356996..1358030	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
H7R04_RS06565	1358243..1358956	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
H7R04_RS06570	1359110..1359337	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
H7R04_RS06575	1359337..1359735	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
H7R04_RS06580	1359882..1360745	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
H7R04_RS06585	1360760..1362775	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
H7R04_RS06590	1362849..1363547	+	699	WP_000679823.1	esterase	-
H7R04_RS06595	1363657..1363857	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T34620 WP_000911330.1 NZ_AP022120:1359337-1359735 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T34620 NZ_AP022120:1359337-1359735 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT34620 WP_000450524.1 NZ_AP022120:1359110-1359337 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT34620 NZ_AP022120:1359110-1359337 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33