Detailed information of TA system TA034615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 16887..17530
• Replicon: plasmid pWP5-W18-ESBL-06_3
• Accession: NZ_AP022115
• Organism: Klebsiella quasipneumoniae strain WP5-W18-ESBL-06

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: H7R83_RS28400
• Protein ID: WP_001044770.1
• Coordinates: 16887..17303 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: H7R83_RS28405
• Protein ID: WP_001261282.1
• Coordinates: 17300..17530 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R83_RS28370	12232..12789	+	558	WP_001217881.1	recombinase family protein	-
H7R83_RS28375	13023..13577	+	555	WP_063840280.1	AAC(6')-Ib family aminoglycoside 6'-N-acetyltransferase	-
H7R83_RS28380	13647..14435	+	789	WP_001206315.1	ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1	-
H7R83_RS28385	14495..15319	+	825	Protein_16	class D beta-lactamase	-
H7R83_RS28390	15372..16069	-	698	Protein_17	IS1 family transposase	-
H7R83_RS28395	16103..16813	-	711	WP_004152334.1	ATP-binding protein	-
H7R83_RS28400	16887..17303	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
H7R83_RS28405	17300..17530	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H7R83_RS28410	17487..17947	+	461	Protein_21	hypothetical protein	-
H7R83_RS28415	18075..18395	+	321	WP_004197770.1	hypothetical protein	-
H7R83_RS28420	18444..19265	+	822	WP_073553765.1	hypothetical protein	-
H7R83_RS28425	19262..20044	+	783	WP_048268853.1	site-specific integrase	-
H7R83_RS28430	20467..21177	+	711	WP_073553760.1	hypothetical protein	-
H7R83_RS28435	21249..21698	+	450	WP_073553761.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaSHV-12 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9	-	1..107360	107360

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T34615 WP_001044770.1 NZ_AP022115:c17303-16887 [Klebsiella quasipneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T34615 NZ_AP022115:c17303-16887 [Klebsiella quasipneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT34615 WP_001261282.1 NZ_AP022115:c17530-17300 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT34615 NZ_AP022115:c17530-17300 [Klebsiella quasipneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3