Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 139207..139943 Replicon plasmid pWP5-W18-ESBL-06_1
Accession NZ_AP022113
Organism Klebsiella quasipneumoniae strain WP5-W18-ESBL-06

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag H7R83_RS27460 Protein ID WP_003026803.1
Coordinates 139461..139943 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag H7R83_RS27455 Protein ID WP_003026799.1
Coordinates 139207..139473 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R83_RS27430 134539..134847 - 309 WP_017896554.1 hypothetical protein -
H7R83_RS27435 134987..136009 + 1023 WP_001567660.1 IS110 family transposase -
H7R83_RS27445 137933..138481 + 549 WP_001567366.1 thioredoxin fold domain-containing protein -
H7R83_RS27450 138528..138962 + 435 WP_001567367.1 cell envelope integrity protein TolA -
H7R83_RS27455 139207..139473 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
H7R83_RS27460 139461..139943 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
H7R83_RS27465 140144..141547 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
H7R83_RS27470 141576..142208 - 633 WP_001567369.1 hypothetical protein -
H7R83_RS27475 142732..143538 - 807 Protein_160 IS110-like element IS4321 family transposase -
H7R83_RS27480 143602..144306 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
H7R83_RS27485 144590..144880 + 291 WP_001247892.1 nucleotidyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..190538 190538
- inside IScluster/Tn - - 134987..150408 15421


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T34614 WP_003026803.1 NZ_AP022113:139461-139943 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T34614 NZ_AP022113:139461-139943 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT34614 WP_003026799.1 NZ_AP022113:139207-139473 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT34614 NZ_AP022113:139207-139473 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATTCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References