Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 105294..105820 Replicon plasmid pWP5-W18-ESBL-06_1
Accession NZ_AP022113
Organism Klebsiella quasipneumoniae strain WP5-W18-ESBL-06

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H7R83_RS27255 Protein ID WP_000323025.1
Coordinates 105533..105820 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H7R83_RS27250 Protein ID WP_000534858.1
Coordinates 105294..105533 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R83_RS27235 101622..102860 + 1239 WP_003030308.1 IS110 family transposase -
H7R83_RS27240 103336..103908 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
H7R83_RS27245 104108..105031 + 924 WP_020277922.1 cation diffusion facilitator family transporter -
H7R83_RS27250 105294..105533 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H7R83_RS27255 105533..105820 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H7R83_RS27260 105887..106921 - 1035 Protein_117 IS481 family transposase -
H7R83_RS27265 106982..107143 + 162 WP_032426744.1 type I toxin-antitoxin system Hok family toxin -
H7R83_RS27270 107304..107789 - 486 WP_032752057.1 hypothetical protein -
H7R83_RS27275 107979..108251 + 273 WP_023280873.1 hypothetical protein -
H7R83_RS27280 108248..108598 + 351 WP_020277938.1 hypothetical protein -
H7R83_RS27285 109115..109495 + 381 WP_015632488.1 hypothetical protein -
H7R83_RS27290 109561..109908 + 348 WP_020316654.1 hypothetical protein -
H7R83_RS27295 109996..110226 + 231 WP_020805755.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..190538 190538


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T34613 WP_000323025.1 NZ_AP022113:105533-105820 [Klebsiella quasipneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T34613 NZ_AP022113:105533-105820 [Klebsiella quasipneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT34613 WP_000534858.1 NZ_AP022113:105294-105533 [Klebsiella quasipneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT34613 NZ_AP022113:105294-105533 [Klebsiella quasipneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References