Detailed information of TA system TA034611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 56144..56787
• Replicon: plasmid pWP5-W18-ESBL-06_1
• Accession: NZ_AP022113
• Organism: Klebsiella quasipneumoniae strain WP5-W18-ESBL-06

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: H7R83_RS26970
• Protein ID: WP_001044770.1
• Coordinates: 56144..56560 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: H7R83_RS26975
• Protein ID: WP_001261282.1
• Coordinates: 56557..56787 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R83_RS26950	51484..52885	+	1402	Protein_55	sucrose-6-phosphate hydrolase	-
H7R83_RS26955	52915..53919	+	1005	WP_136392550.1	LacI family DNA-binding transcriptional regulator	-
H7R83_RS26960	54303..55000	-	698	Protein_57	IS1 family transposase	-
H7R83_RS26965	55057..56070	-	1014	Protein_58	AAA family ATPase	-
H7R83_RS26970	56144..56560	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
H7R83_RS26975	56557..56787	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H7R83_RS26980	56744..57205	+	462	WP_162574861.1	hypothetical protein	-
H7R83_RS26985	57375..57593	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	-
H7R83_RS26990	57595..57900	+	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	-
H7R83_RS26995	58129..58269	+	141	WP_162898808.1	hypothetical protein	-
H7R83_RS27000	58319..58654	+	336	WP_009309920.1	hypothetical protein	-
H7R83_RS27005	58688..59704	+	1017	WP_009309921.1	hypothetical protein	-
H7R83_RS27010	59902..60681	+	780	WP_023287113.1	site-specific integrase	-
H7R83_RS27015	60739..60996	-	258	WP_009310077.1	hypothetical protein	-
H7R83_RS27020	61125..61238	-	114	WP_014343462.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..190538	190538
	-	flank	IS/Tn	-	-	54303..54806	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T34611 WP_001044770.1 NZ_AP022113:c56560-56144 [Klebsiella quasipneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T34611 NZ_AP022113:c56560-56144 [Klebsiella quasipneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT34611 WP_001261282.1 NZ_AP022113:c56787-56557 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT34611 NZ_AP022113:c56787-56557 [Klebsiella quasipneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3