Detailed information of TA system TA034595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 319890..320476
• Replicon: chromosome
• Accession: NZ_AP022112
• Organism: Klebsiella quasipneumoniae strain WP5-W18-ESBL-06

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: H7R83_RS01470
• Protein ID: WP_002920800.1
• Coordinates: 320108..320476 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: H7R83_RS01465
• Protein ID: WP_048295677.1
• Coordinates: 319890..320111 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R83_RS01445	316047..316973	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
H7R83_RS01450	316970..318247	+	1278	WP_004202111.1	branched chain amino acid ABC transporter permease LivM	-
H7R83_RS01455	318244..319011	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
H7R83_RS01460	319013..319726	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
H7R83_RS01465	319890..320111	+	222	WP_048295677.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H7R83_RS01470	320108..320476	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
H7R83_RS01475	320768..322084	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
H7R83_RS01480	322191..323078	+	888	WP_004202113.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
H7R83_RS01485	323075..323920	+	846	WP_023319266.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
H7R83_RS01490	323922..324992	+	1071	WP_017899977.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	316970..325729	8759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T34595 WP_002920800.1 NZ_AP022112:320108-320476 [Klebsiella quasipneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T34595 NZ_AP022112:320108-320476 [Klebsiella quasipneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCATGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCTGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8135.20 Da
• Isoelectric Point: 5.0637

>AT34595 WP_048295677.1 NZ_AP022112:319890-320111 [Klebsiella quasipneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGVEIRELADK

Nucleotide

• Length: 222 bp

>AT34595 NZ_AP022112:319890-320111 [Klebsiella quasipneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGTTGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1