Detailed information of TA system TA034594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 140837..141393
• Replicon: plasmid pWP5-W18-ESBL-02_1
• Accession: NZ_AP022111
• Organism: Aeromonas caviae strain WP5-W18-ESBL-02

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: H7R67_RS23220
• Protein ID: WP_182936448.1
• Coordinates: 140837..141112 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H7R67_RS23225
• Protein ID: WP_182936447.1
• Coordinates: 141112..141393 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R67_RS23195	135906..136211	+	306	WP_005320934.1	hypothetical protein	-
H7R67_RS23200	136313..137716	-	1404	WP_182959202.1	IS66-like element ISAs21 family transposase	-
H7R67_RS23205	137803..139056	+	1254	WP_042650430.1	IS256-like element ISAeme25 family transposase	-
H7R67_RS23210	139440..139763	-	324	Protein_134	IS30 family transposase	-
H7R67_RS23215	140059..140787	-	729	WP_182936449.1	carbonic anhydrase	-
H7R67_RS23220	140837..141112	-	276	WP_182936448.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R67_RS23225	141112..141393	-	282	WP_182936447.1	ribbon-helix-helix domain-containing protein	Antitoxin
H7R67_RS23230	141651..142211	+	561	WP_001161490.1	recombinase family protein	-
H7R67_RS23235	142215..145181	+	2967	WP_001138014.1	Tn3-like element TnAs1 family transposase	-
H7R67_RS23240	145178..145783	-	606	WP_182959078.1	replication initiation protein	-
H7R67_RS23245	146191..146388	-	198	WP_182959080.1	chromosome partitioning protein ParB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..147986	147986
	-	inside	IScluster/Tn	-	-	136313..145181	8868

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-50)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10676.43 Da
• Isoelectric Point: 9.5291

>T34594 WP_182936448.1 NZ_AP022111:c141112-140837 [Aeromonas caviae]
MELKWTSKALSDLTRLYEFLALANKPAAARVVQALTQAPAILLTNPRIGEQLFQFEPREVRRMLVGEYEVRYELLDSSIY
ILRLWHTREAR

Nucleotide

• Length: 276 bp

>T34594 NZ_AP022111:c141112-140837 [Aeromonas caviae]
ATGGAACTGAAGTGGACCAGCAAAGCGCTCTCTGATTTGACACGCTTGTATGAATTTTTGGCTCTGGCTAACAAACCCGC
CGCGGCTCGGGTAGTGCAGGCACTGACACAGGCCCCAGCTATTCTGCTAACAAACCCGCGTATTGGAGAACAACTGTTCC
AATTCGAGCCCCGCGAGGTGAGGAGGATGTTGGTTGGAGAATACGAGGTTCGTTACGAGCTCCTTGATTCGAGCATCTAC
ATTCTGCGGCTGTGGCATACCCGAGAAGCTCGTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10368.83 Da
• Isoelectric Point: 5.6706

>AT34594 WP_182936447.1 NZ_AP022111:c141393-141112 [Aeromonas caviae]
MKSNMTQATTKVLTAHVPLPMADKVDQMAARLERSRGWIMKQALSAWIAQEEDRDRLTQEALADVEAGRVIDHLAVQAWA
DSLSTDQPLSVPR

Nucleotide

• Length: 282 bp

>AT34594 NZ_AP022111:c141393-141112 [Aeromonas caviae]
ATGAAGAGCAACATGACACAAGCAACAACCAAGGTGCTTACTGCACACGTACCACTACCTATGGCCGACAAAGTTGACCA
GATGGCAGCCCGCCTTGAACGCTCACGGGGCTGGATCATGAAACAGGCTCTTTCCGCATGGATCGCCCAGGAAGAGGATC
GTGATCGCCTGACCCAAGAGGCGCTGGCAGACGTGGAAGCTGGTCGAGTTATCGATCACTTGGCGGTGCAGGCTTGGGCT
GACAGCCTCAGTACTGACCAGCCGCTGTCGGTTCCGCGTTGA