Detailed information of TA system TA034587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 196940..197490
• Replicon: plasmid pWP5-W18-CRE-01_1
• Accession: NZ_AP022109
• Organism: Klebsiella aerogenes strain WP5-W18-CRE-01

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: H7R63_RS25205
• Protein ID: WP_007372286.1
• Coordinates: 197182..197490 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: H7R63_RS25200
• Protein ID: WP_007372285.1
• Coordinates: 196940..197179 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R63_RS25155	191947..192342	+	396	WP_024196081.1	hypothetical protein	-
H7R63_RS25160	192524..192715	+	192	WP_016241560.1	hypothetical protein	-
H7R63_RS25165	192846..193541	+	696	WP_007372278.1	hypothetical protein	-
H7R63_RS25170	193564..193761	+	198	WP_032155220.1	Lar family restriction alleviation protein	-
H7R63_RS25175	193814..194272	+	459	WP_035942286.1	hypothetical protein	-
H7R63_RS25180	194420..194560	+	141	WP_007372281.1	hypothetical protein	-
H7R63_RS25185	194576..195208	+	633	WP_007372282.1	hypothetical protein	-
H7R63_RS25190	195669..196340	+	672	WP_007372283.1	hypothetical protein	-
H7R63_RS25195	196337..196801	+	465	WP_008786596.1	hypothetical protein	-
H7R63_RS25200	196940..197179	+	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H7R63_RS25205	197182..197490	+	309	WP_007372286.1	CcdB family protein	Toxin
H7R63_RS25210	197511..197669	+	159	WP_016241558.1	hypothetical protein	-
H7R63_RS25215	197824..198378	+	555	WP_007372288.1	hypothetical protein	-
H7R63_RS25220	198448..199065	+	618	WP_007372289.1	hypothetical protein	-
H7R63_RS25225	199148..199618	+	471	WP_007372290.1	hypothetical protein	-
H7R63_RS25230	199965..200207	+	243	WP_007372291.1	hypothetical protein	-
H7R63_RS25235	200218..200616	+	399	WP_007372292.1	hypothetical protein	-
H7R63_RS25240	200700..201026	+	327	WP_007372293.1	hypothetical protein	-
H7R63_RS25245	201389..201727	-	339	WP_007372294.1	hypothetical protein	-
H7R63_RS25250	201965..202408	+	444	WP_007372295.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	formA	-	1..326767	326767

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11351.17 Da
• Isoelectric Point: 8.5044

>T34587 WP_007372286.1 NZ_AP022109:197182-197490 [Klebsiella aerogenes]
MQYTVYRNPGNSQAYPYLLDIQSDIIGELNTRLVIPLHRLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKSAVDFLLDGF

Nucleotide

• Length: 309 bp

>T34587 NZ_AP022109:197182-197490 [Klebsiella aerogenes]
ATGCAATATACCGTTTACCGCAATCCCGGCAACAGCCAGGCATACCCTTACCTGCTGGATATACAGAGCGACATCATTGG
CGAGTTAAATACCCGCCTGGTGATCCCCCTTCATCGGCTTAAAAAAGGAGCCTCGGCCCCGGTTGCGCGATTAACTCCGG
TCATACAGGTGGAAGGGAATGACGTTATCCTGATGACGCATGAAATGGCTTCAGTCCGCGTGAAGCAGCTCGGTCAGGCG
GTCATGGACGCATCTCCGTTCCGGCACACTATCAAAAGTGCGGTGGACTTCCTGCTGGATGGTTTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8784.97 Da
• Isoelectric Point: 8.6387

>AT34587 WP_007372285.1 NZ_AP022109:196940-197179 [Klebsiella aerogenes]
MQATAARQKKTVSVTLEPMLVQQAKDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Nucleotide

• Length: 240 bp

>AT34587 NZ_AP022109:196940-197179 [Klebsiella aerogenes]
ATGCAGGCTACAGCTGCTCGCCAGAAAAAAACCGTCAGTGTCACCCTTGAGCCAATGCTCGTACAGCAGGCAAAAGACGC
TGGGATTAACTTGTCCGCCACGCTGGCAGAAGCGCTTCAGAGCAAACTGAAGGCCAGTGCAGCGGAAGAATGGAAACGAC
AGAATCGTGCTGGTATTCAGGAGTTGAACCGCATCACTGAAGAGCATGGGTTACTGTCTGACGAATACAGGACGTTCTGA

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3