Detailed information of TA system TA034586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4519323..4520133
• Replicon: chromosome
• Accession: NZ_AP022108
• Organism: Klebsiella aerogenes strain WP5-W18-CRE-01

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: H7R63_RS21265
• Protein ID: WP_032715534.1
• Coordinates: 4519323..4519856 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: H7R63_RS21270
• Protein ID: WP_002887278.1
• Coordinates: 4519867..4520133 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R63_RS21260	4518155..4519276	+	1122	WP_015704249.1	cupin domain-containing protein	-
H7R63_RS21265	4519323..4519856	-	534	WP_032715534.1	GNAT family N-acetyltransferase	Toxin
H7R63_RS21270	4519867..4520133	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
H7R63_RS21275	4520212..4521669	-	1458	WP_032715533.1	Cu(+)/Ag(+) sensor histidine kinase	-
H7R63_RS21280	4521659..4522342	-	684	WP_032715532.1	copper response regulator transcription factor CusR	-
H7R63_RS21285	4522516..4523901	+	1386	WP_032715531.1	efflux transporter outer membrane subunit	-
H7R63_RS21290	4523919..4524263	+	345	WP_032715529.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19908.80 Da
• Isoelectric Point: 5.2753

>T34586 WP_032715534.1 NZ_AP022108:c4519856-4519323 [Klebsiella aerogenes]
MDQLLTIEMIADEFRYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T34586 NZ_AP022108:c4519856-4519323 [Klebsiella aerogenes]
ATGGATCAGCTACTGACGATTGAGATGATTGCCGATGAGTTCCGCTACGACATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAACATCTCAAGCGACAGCACGATGGACAGATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGCTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGCGGCATGCTGCCGTCTAAAACTCAG
CAAAAGAAAATTCCGTATCAAAATGCGCCAAGCGTAACTCTGGGGCGGCTGGCCATTGATAAATCCGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTCGCCCATGCGATGCGAGTGGTGTGGGGGGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCTCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGATGATGAGTCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT34586 WP_002887278.1 NZ_AP022108:c4520133-4519867 [Klebsiella aerogenes]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT34586 NZ_AP022108:c4520133-4519867 [Klebsiella aerogenes]
ATGCCCGCACTCAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACTCAACCGCCAGCACCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGATAA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1