Detailed information of TA system TA034584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2592021..2592760
• Replicon: chromosome
• Accession: NZ_AP022108
• Organism: Klebsiella aerogenes strain WP5-W18-CRE-01

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: H7R63_RS12265
• Protein ID: WP_182940570.1
• Coordinates: 2592275..2592760 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A0H3FSM9
• Locus tag: H7R63_RS12260
• Protein ID: WP_015705366.1
• Coordinates: 2592021..2592287 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R63_RS12240	2588314..2588931	+	618	WP_032714468.1	response regulator transcription factor	-
H7R63_RS12245	2589027..2589524	+	498	WP_032714467.1	heme-binding protein	-
H7R63_RS12250	2589928..2590458	+	531	WP_032714466.1	N-acetyltransferase	-
H7R63_RS12255	2590513..2591748	-	1236	WP_182940568.1	MFS transporter	-
H7R63_RS12260	2592021..2592287	+	267	WP_015705366.1	DUF1778 domain-containing protein	Antitoxin
H7R63_RS12265	2592275..2592760	+	486	WP_182940570.1	GNAT family N-acetyltransferase	Toxin
H7R63_RS12270	2592832..2594448	+	1617	WP_182940572.1	FAD-NAD(P)-binding protein	-
H7R63_RS12275	2594567..2595667	+	1101	WP_032714465.1	NADH:flavin oxidoreductase/NADH oxidase	-
H7R63_RS12280	2595724..2595882	-	159	WP_002903230.1	YqaE/Pmp3 family membrane protein	-
H7R63_RS12285	2596143..2597213	+	1071	WP_032714463.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17575.20 Da
• Isoelectric Point: 8.5155

>T34584 WP_182940570.1 NZ_AP022108:2592275-2592760 [Klebsiella aerogenes]
MGKISAPAPLSSHHQIAEFCCGETVLDQWLKQRGLKNQAQGAARTFVVCKEESHQVVGFYSLATGSVNHTEATGGLRRNM
PDPIPVIILARLAIDCAYHGQGLGADLLHDALLRSYRVAENVGVRALMVHALTDSAKQFYLHHGFKASTTQERTLFLALP
K

Nucleotide

• Length: 486 bp

>T34584 NZ_AP022108:2592275-2592760 [Klebsiella aerogenes]
ATGGGAAAAATAAGCGCGCCTGCACCGCTATCTTCACACCATCAGATCGCGGAATTTTGTTGTGGAGAAACGGTTCTCGA
TCAATGGCTGAAACAGCGAGGATTGAAAAACCAGGCTCAGGGCGCCGCGAGAACCTTTGTGGTCTGCAAGGAAGAGAGCC
ATCAGGTGGTGGGGTTTTATTCGTTAGCAACCGGCAGCGTTAATCACACGGAAGCCACTGGCGGCCTGCGGCGCAATATG
CCCGATCCTATCCCGGTCATCATTCTCGCCCGCCTGGCGATCGACTGCGCGTATCACGGACAGGGGCTGGGAGCTGATTT
ACTCCATGATGCGCTCTTACGCAGTTATCGCGTTGCCGAAAATGTTGGCGTTCGTGCCCTGATGGTCCATGCCCTGACCG
ATAGCGCGAAACAATTCTATTTACACCACGGATTCAAAGCCTCCACCACCCAGGAACGCACACTTTTCCTCGCGTTGCCT
AAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10085.56 Da
• Isoelectric Point: 4.6558

>AT34584 WP_015705366.1 NZ_AP022108:2592021-2592287 [Klebsiella aerogenes]
MKSDIQLNIRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNLSDEQYAEFIDMLDAPVEANPALDKLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT34584 NZ_AP022108:2592021-2592287 [Klebsiella aerogenes]
ATGAAATCAGATATCCAACTCAACATCCGGGCGAAGGAGTCCCAGCGGGCGCTGATTGATGCGGCTGCCGAAATTCTGCA
CAAATCACGGACCGACTTTATTCTTGAGATGGCCTGCCAGGCGGCAGAAAACGTCATTCTCGACCGCCGGGTGTTTAACT
TAAGCGATGAGCAATACGCCGAATTTATCGACATGCTGGATGCGCCGGTTGAAGCTAACCCCGCTCTCGATAAATTGCTG
GCGAGAAAACCACAATGGGAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3FSM9