Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4041742..4042318 Replicon chromosome
Accession NZ_AP022107
Organism Escherichia coli strain WP5-S17-ESBL-11

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag H7K47_RS19350 Protein ID WP_001295743.1
Coordinates 4042031..4042318 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A6N8Q8R5
Locus tag H7K47_RS19345 Protein ID WP_001422870.1
Coordinates 4041742..4042044 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7K47_RS19330 4038338..4040488 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
H7K47_RS19335 4040538..4040741 + 204 WP_000467859.1 YbdD/YjiX family protein -
H7K47_RS19340 4040752..4041708 + 957 WP_001295745.1 GTPase -
H7K47_RS19345 4041742..4042044 - 303 WP_001422870.1 BrnA antitoxin family protein Antitoxin
H7K47_RS19350 4042031..4042318 - 288 WP_001295743.1 BrnT family toxin Toxin
H7K47_RS19355 4042515..4043681 + 1167 WP_000800831.1 restriction endonuclease -
H7K47_RS19360 4043745..4045364 + 1620 WP_001029745.1 type I restriction-modification system subunit M -
H7K47_RS19365 4045354..4046658 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T34570 WP_001295743.1 NZ_AP022107:c4042318-4042031 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T34570 NZ_AP022107:c4042318-4042031 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11582.09 Da        Isoelectric Point: 9.4646

>AT34570 WP_001422870.1 NZ_AP022107:c4042044-4041742 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRSGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT34570 NZ_AP022107:c4042044-4041742 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGATCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A6N8Q8R5

References