Detailed information of TA system TA034568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3505782..3506619
• Replicon: chromosome
• Accession: NZ_AP022107
• Organism: Escherichia coli strain WP5-S17-ESBL-11

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: H7K47_RS16790
• Protein ID: WP_000227784.1
• Coordinates: 3506077..3506619 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: H7K47_RS16785
• Protein ID: WP_001297137.1
• Coordinates: 3505782..3506093 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7K47_RS16760	3500802..3501749	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
H7K47_RS16765	3501771..3503762	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
H7K47_RS16770	3503752..3504366	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
H7K47_RS16775	3504366..3504695	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
H7K47_RS16780	3504707..3505597	+	891	WP_000971336.1	heme o synthase	-
H7K47_RS16785	3505782..3506093	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
H7K47_RS16790	3506077..3506619	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
H7K47_RS16795	3506675..3507610	-	936	WP_001297127.1	sel1 repeat family protein	-
H7K47_RS16800	3508018..3509382	+	1365	WP_001000978.1	MFS transporter	-
H7K47_RS16805	3509510..3510001	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
H7K47_RS16810	3510169..3511080	+	912	WP_000705847.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T34568 WP_000227784.1 NZ_AP022107:3506077-3506619 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T34568 NZ_AP022107:3506077-3506619 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT34568 WP_001297137.1 NZ_AP022107:3505782-3506093 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT34568 NZ_AP022107:3505782-3506093 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3