Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2461538..2462176 Replicon chromosome
Accession NZ_AP022107
Organism Escherichia coli strain WP5-S17-ESBL-11

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag H7K47_RS11775 Protein ID WP_000813794.1
Coordinates 2462000..2462176 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag H7K47_RS11770 Protein ID WP_001270286.1
Coordinates 2461538..2461954 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7K47_RS11750 2456690..2457631 - 942 WP_001251319.1 ABC transporter permease -
H7K47_RS11755 2457632..2458645 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
H7K47_RS11760 2458663..2459808 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
H7K47_RS11765 2460053..2461459 - 1407 WP_001421323.1 PLP-dependent aminotransferase family protein -
H7K47_RS11770 2461538..2461954 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
H7K47_RS11775 2462000..2462176 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
H7K47_RS11780 2462398..2462628 + 231 WP_000494244.1 YncJ family protein -
H7K47_RS11785 2462720..2464681 - 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
H7K47_RS11790 2464754..2465290 - 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
H7K47_RS11795 2465343..2466557 + 1215 WP_001349372.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2466597..2467862 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T34565 WP_000813794.1 NZ_AP022107:c2462176-2462000 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T34565 NZ_AP022107:c2462176-2462000 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT34565 WP_001270286.1 NZ_AP022107:c2461954-2461538 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT34565 NZ_AP022107:c2461954-2461538 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References