Detailed information of TA system TA034555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1300428..1301053
• Replicon: chromosome
• Accession: NZ_AP022107
• Organism: Escherichia coli strain WP5-S17-ESBL-11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H7K47_RS06255
• Protein ID: WP_000911330.1
• Coordinates: 1300655..1301053 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: H7K47_RS06250
• Protein ID: WP_000450524.1
• Coordinates: 1300428..1300655 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7K47_RS06225	1296231..1296701	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
H7K47_RS06230	1296701..1297273	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
H7K47_RS06235	1297419..1298297	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
H7K47_RS06240	1298314..1299348	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
H7K47_RS06245	1299561..1300274	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
H7K47_RS06250	1300428..1300655	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
H7K47_RS06255	1300655..1301053	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
H7K47_RS06260	1301200..1302063	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
H7K47_RS06265	1302078..1304093	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
H7K47_RS06270	1304167..1304865	+	699	WP_000679823.1	esterase	-
H7K47_RS06275	1304975..1305175	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T34555 WP_000911330.1 NZ_AP022107:1300655-1301053 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T34555 NZ_AP022107:1300655-1301053 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT34555 WP_000450524.1 NZ_AP022107:1300428-1300655 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT34555 NZ_AP022107:1300428-1300655 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33