34542

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 38760..39285 Replicon plasmid pWP5-S17-ESBL-10_1
Accession NZ_AP022106
Organism Escherichia coli strain WP5-S17-ESBL-10

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H7R00_RS23615 Protein ID WP_001159871.1
Coordinates 38980..39285 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag H7R00_RS23610 Protein ID WP_000813630.1
Coordinates 38760..38978 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R00_RS23585 33970..34656 + 687 Protein_36 IS1 family transposase -
H7R00_RS23590 34910..35932 - 1023 WP_000361402.1 helicase UvrD -
H7R00_RS23595 35917..37482 - 1566 WP_001128474.1 AAA family ATPase -
H7R00_RS23600 37557..37973 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
H7R00_RS23605 37970..38200 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H7R00_RS23610 38760..38978 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R00_RS23615 38980..39285 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R00_RS23620 39286..40092 + 807 WP_000016970.1 site-specific integrase -
H7R00_RS23625 40814..41569 + 756 WP_000852146.1 replication initiation protein RepE -
H7R00_RS23630 42157..43323 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B iucA / iucB / iucC / iucD / iutA / senB 1..130869 130869
- flank IS/Tn - - 34309..34656 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T34542 WP_001159871.1 NZ_AP022106:38980-39285 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T34542 NZ_AP022106:38980-39285 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT34542 WP_000813630.1 NZ_AP022106:38760-38978 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT34542 NZ_AP022106:38760-38978 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References