Detailed information of TA system TA034538

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4199720..4200554
• Replicon: chromosome
• Accession: NZ_AP022105
• Organism: Escherichia coli strain WP5-S17-ESBL-10

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: H7R00_RS20190
• Protein ID: WP_000854770.1
• Coordinates: 4199720..4200097 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M1N885
• Locus tag: H7R00_RS20195
• Protein ID: WP_001280950.1
• Coordinates: 4200186..4200554 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R00_RS20165	4195831..4197471	-	1641	WP_115776216.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-
H7R00_RS20170	4198244..4198420	-	177	Protein_3948	helix-turn-helix domain-containing protein	-
H7R00_RS20175	4198784..4198942	-	159	WP_001467148.1	hypothetical protein	-
H7R00_RS20180	4199042..4199218	-	177	WP_000839286.1	DUF957 domain-containing protein	-
H7R00_RS20185	4199235..4199723	-	489	WP_000761690.1	hypothetical protein	-
H7R00_RS20190	4199720..4200097	-	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
H7R00_RS20195	4200186..4200554	-	369	WP_001280950.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H7R00_RS20200	4200717..4200938	-	222	WP_001220314.1	DUF987 domain-containing protein	-
H7R00_RS20205	4201001..4201477	-	477	WP_001186779.1	RadC family protein	-
H7R00_RS20210	4201493..4201957	-	465	WP_115776217.1	antirestriction protein	-
H7R00_RS20215	4202299..4203117	-	819	WP_001234712.1	DUF945 domain-containing protein	-
H7R00_RS20220	4203272..4203430	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T34538 WP_000854770.1 NZ_AP022105:c4200097-4199720 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T34538 NZ_AP022105:c4200097-4199720 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13737.56 Da
• Isoelectric Point: 7.0268

>AT34538 WP_001280950.1 NZ_AP022105:c4200554-4200186 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT34538 NZ_AP022105:c4200554-4200186 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9

Antitoxin

• AlphaFold DB: A0A1M1N885