Detailed information of TA system TA034537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4168351..4169171
• Replicon: chromosome
• Accession: NZ_AP022105
• Organism: Escherichia coli strain WP5-S17-ESBL-10

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: H7R00_RS20040
• Protein ID: WP_001054379.1
• Coordinates: 4168351..4168608 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: H7R00_RS20045
• Protein ID: WP_000123957.1
• Coordinates: 4168620..4169171 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R00_RS20015	4164910..4165191	-	282	Protein_3916	hypothetical protein	-
H7R00_RS20020	4165289..4166068	-	780	WP_001297096.1	IS21-like element IS100kyp family helper ATPase IstB	-
H7R00_RS20025	4166068..4167090	-	1023	WP_000255956.1	IS21-like element IS100 family transposase	-
H7R00_RS20030	4167178..4167597	-	420	Protein_3919	DNA helicase	-
H7R00_RS20035	4167675..4167974	+	300	WP_115776213.1	GNAT family N-acetyltransferase	-
H7R00_RS20040	4168351..4168608	+	258	WP_001054379.1	YjhX family toxin	Toxin
H7R00_RS20045	4168620..4169171	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
H7R00_RS20050	4169223..4169969	+	747	WP_115776214.1	class I SAM-dependent methyltransferase	-
H7R00_RS20055	4170097..4170357	+	261	WP_000077645.1	hypothetical protein	-
H7R00_RS24200	4170395..4170511	+	117	Protein_3925	VOC family protein	-
H7R00_RS20060	4170756..4171877	+	1122	WP_000010833.1	M42 family metallopeptidase	-
H7R00_RS20065	4171874..4172152	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
H7R00_RS20070	4172164..4173477	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T34537 WP_001054379.1 NZ_AP022105:4168351-4168608 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T34537 NZ_AP022105:4168351-4168608 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT34537 WP_000123957.1 NZ_AP022105:4168620-4169171 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT34537 NZ_AP022105:4168620-4169171 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030