34536

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4126905..4127481 Replicon chromosome
Accession NZ_AP022105
Organism Escherichia coli strain WP5-S17-ESBL-10

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag H7R00_RS19820 Protein ID WP_001297643.1
Coordinates 4127194..4127481 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag H7R00_RS19815 Protein ID WP_000063156.1
Coordinates 4126905..4127207 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R00_RS19800 4123501..4125651 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
H7R00_RS19805 4125701..4125904 + 204 WP_000467859.1 YbdD/YjiX family protein -
H7R00_RS19810 4125915..4126871 + 957 WP_001297640.1 GTPase -
H7R00_RS19815 4126905..4127207 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
H7R00_RS19820 4127194..4127481 - 288 WP_001297643.1 BrnT family toxin Toxin
H7R00_RS19825 4127680..4128377 - 698 Protein_3878 IS1 family transposase -
H7R00_RS19830 4128434..4128529 + 96 Protein_3879 dienelactone hydrolase family protein -
H7R00_RS19835 4128675..4129907 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
H7R00_RS19840 4129948..4131228 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T34536 WP_001297643.1 NZ_AP022105:c4127481-4127194 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T34536 NZ_AP022105:c4127481-4127194 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT34536 WP_000063156.1 NZ_AP022105:c4127207-4126905 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT34536 NZ_AP022105:c4127207-4126905 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References