Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4209584..4210134 Replicon chromosome
Accession NZ_AP022098
Organism Escherichia coli strain WP4-S18-ESBL-09

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag H7R19_RS20120 Protein ID WP_001160966.1
Coordinates 4209584..4209898 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag H7R19_RS20125 Protein ID WP_000125568.1
Coordinates 4209901..4210134 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R19_RS20095 4205259..4206545 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
H7R19_RS20100 4206545..4207534 + 990 WP_000241224.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
H7R19_RS20105 4207531..4208352 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
H7R19_RS20110 4208355..4208732 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
H7R19_RS20115 4208739..4209587 + 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
H7R19_RS20120 4209584..4209898 - 315 WP_001160966.1 CcdB family protein Toxin
H7R19_RS20125 4209901..4210134 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
H7R19_RS20130 4210220..4210699 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
H7R19_RS20135 4210890..4212752 - 1863 WP_022645165.1 glutathione-regulated potassium-efflux system protein KefC -
H7R19_RS20140 4212745..4213275 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
H7R19_RS20145 4213383..4214714 - 1332 WP_001183201.1 MFS transporter -
H7R19_RS20150 4214772..4215059 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T34511 WP_001160966.1 NZ_AP022098:c4209898-4209584 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T34511 NZ_AP022098:c4209898-4209584 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCACACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT34511 WP_000125568.1 NZ_AP022098:c4210134-4209901 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT34511 NZ_AP022098:c4210134-4209901 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References