Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 37436..37961 Replicon plasmid pWP4-S18-ESBL-08_1
Accession NZ_AP022096
Organism Escherichia coli strain WP4-S18-ESBL-08

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag H7R08_RS23830 Protein ID WP_001159868.1
Coordinates 37656..37961 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag H7R08_RS23825 Protein ID WP_000813634.1
Coordinates 37436..37654 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R08_RS23795 32828..33244 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
H7R08_RS23800 33241..33471 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H7R08_RS23805 33736..34236 + 501 WP_000528932.1 hypothetical protein -
H7R08_RS23810 34249..35022 + 774 WP_000905949.1 hypothetical protein -
H7R08_RS23815 35189..36322 + 1134 WP_000545984.1 DUF3800 domain-containing protein -
H7R08_RS23820 36356..36868 - 513 WP_000151784.1 hypothetical protein -
H7R08_RS23825 37436..37654 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R08_RS23830 37656..37961 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R08_RS23835 37962..38768 + 807 WP_000016982.1 site-specific integrase -
H7R08_RS23840 39520..40275 + 756 WP_000852146.1 replication initiation protein RepE -
H7R08_RS23845 40863..42029 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / blaTEM-1B iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB 1..158292 158292


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T34480 WP_001159868.1 NZ_AP022096:37656-37961 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T34480 NZ_AP022096:37656-37961 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT34480 WP_000813634.1 NZ_AP022096:37436-37654 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT34480 NZ_AP022096:37436-37654 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References