Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 32828..33471 Replicon plasmid pWP4-S18-ESBL-08_1
Accession NZ_AP022096
Organism Escherichia coli strain WP4-S18-ESBL-08

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag H7R08_RS23795 Protein ID WP_001034046.1
Coordinates 32828..33244 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag H7R08_RS23800 Protein ID WP_001261278.1
Coordinates 33241..33471 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R08_RS23780 27965..28381 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
H7R08_RS23785 28378..28608 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H7R08_RS23790 28989..32783 + 3795 WP_001144731.1 hypothetical protein -
H7R08_RS23795 32828..33244 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
H7R08_RS23800 33241..33471 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7R08_RS23805 33736..34236 + 501 WP_000528932.1 hypothetical protein -
H7R08_RS23810 34249..35022 + 774 WP_000905949.1 hypothetical protein -
H7R08_RS23815 35189..36322 + 1134 WP_000545984.1 DUF3800 domain-containing protein -
H7R08_RS23820 36356..36868 - 513 WP_000151784.1 hypothetical protein -
H7R08_RS23825 37436..37654 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
H7R08_RS23830 37656..37961 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / blaTEM-1B iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB 1..158292 158292


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T34479 WP_001034046.1 NZ_AP022096:c33244-32828 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T34479 NZ_AP022096:c33244-32828 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT34479 WP_001261278.1 NZ_AP022096:c33471-33241 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT34479 NZ_AP022096:c33471-33241 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References