Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 968675..969329 Replicon chromosome
Accession NZ_AP022095
Organism Escherichia coli strain WP4-S18-ESBL-08

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag H7R08_RS04640 Protein ID WP_000244783.1
Coordinates 968922..969329 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H7R08_RS04635 Protein ID WP_000354046.1
Coordinates 968675..968941 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R08_RS04610 963844..964587 + 744 WP_000951948.1 SDR family oxidoreductase -
H7R08_RS04615 964644..966077 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
H7R08_RS04620 966122..966433 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
H7R08_RS04625 966597..967256 + 660 WP_000250274.1 hemolysin III family protein -
H7R08_RS04630 967452..968432 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H7R08_RS04635 968675..968941 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H7R08_RS04640 968922..969329 + 408 WP_000244783.1 protein YgfX Toxin
H7R08_RS04645 969369..969890 - 522 WP_001055874.1 flavodoxin FldB -
H7R08_RS04650 970002..970898 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H7R08_RS04655 970923..971633 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H7R08_RS04660 971639..973372 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T34460 WP_000244783.1 NZ_AP022095:968922-969329 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T34460 NZ_AP022095:968922-969329 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTAGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT34460 WP_000354046.1 NZ_AP022095:968675-968941 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT34460 NZ_AP022095:968675-968941 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References