Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 52625..53172 Replicon plasmid pWP4-S18-ESBL-07_4
Accession NZ_AP022091
Organism Escherichia coli strain WP4-S18-ESBL-07

Toxin (Protein)


Gene name parE Uniprot ID A0A4Y1YQH8
Locus tag H7R53_RS25875 Protein ID WP_012881118.1
Coordinates 52891..53172 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E2GHF7
Locus tag H7R53_RS25870 Protein ID WP_012881119.1
Coordinates 52625..52894 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R53_RS25830 48254..48466 + 213 WP_001309245.1 ANR family transcriptional regulator -
H7R53_RS25835 48711..49172 + 462 WP_001233838.1 thermonuclease family protein -
H7R53_RS25840 49218..49427 + 210 WP_001298565.1 hemolysin expression modulator Hha -
H7R53_RS25845 49465..50055 + 591 WP_000766807.1 DUF2726 domain-containing protein -
H7R53_RS25850 50295..50549 + 255 WP_000083830.1 replication regulatory protein RepA -
H7R53_RS25855 50787..50861 + 75 WP_001365705.1 RepA leader peptide Tap -
H7R53_RS25860 50854..51711 + 858 WP_000130646.1 incFII family plasmid replication initiator RepA -
H7R53_RS25865 52412..52570 + 159 WP_032142560.1 hypothetical protein -
H7R53_RS25870 52625..52894 + 270 WP_012881119.1 ABC transporter ATPase Antitoxin
H7R53_RS25875 52891..53172 + 282 WP_012881118.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R53_RS25880 53218..54066 + 849 WP_012881117.1 3'-5' exonuclease -
H7R53_RS25885 54183..54665 + 483 WP_001311056.1 hypothetical protein -
H7R53_RS25890 54700..55806 - 1107 Protein_70 maltoporin LamB -
H7R53_RS25895 56258..57133 - 876 WP_001617865.1 class A extended-spectrum beta-lactamase CTX-M-14 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-14 - 1..74261 74261
- flank IS/Tn blaCTX-M-14 - 56258..58645 2387


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10875.53 Da        Isoelectric Point: 8.4615

>T34456 WP_012881118.1 NZ_AP022091:52891-53172 [Escherichia coli]
MMEIFWTILASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T34456 NZ_AP022091:52891-53172 [Escherichia coli]
ATGATGGAGATATTCTGGACCATACTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTATATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10206.54 Da        Isoelectric Point: 8.8735

>AT34456 WP_012881119.1 NZ_AP022091:52625-52894 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSATAWRD
EISRKVAGK

Download         Length: 270 bp

>AT34456 NZ_AP022091:52625-52894 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCCCATGATATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATAAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Y1YQH8

Antitoxin

Source ID Structure
AlphaFold DB A0A4Y1YSD8

References