Detailed information of TA system TA034455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 24969..25528
• Replicon: plasmid pWP4-S18-ESBL-07_3
• Accession: NZ_AP022090
• Organism: Escherichia coli strain WP4-S18-ESBL-07

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: H7R53_RS25265
• Protein ID: WP_000421257.1
• Coordinates: 24969..25244 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: H7R53_RS25270
• Protein ID: WP_001178087.1
• Coordinates: 25244..25528 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R53_RS25235	20265..20891	+	627	WP_000587689.1	ParA family plasmid-partitioning AAA ATPase	-
H7R53_RS25240	20888..21190	+	303	WP_001245884.1	hypothetical protein	-
H7R53_RS25245	21685..23031	-	1347	WP_001132037.1	hypothetical protein	-
H7R53_RS25250	23385..23987	+	603	WP_000517694.1	hypothetical protein	-
H7R53_RS25255	24125..24391	+	267	WP_000627831.1	hypothetical protein	-
H7R53_RS25260	24582..24863	-	282	WP_001105066.1	hypothetical protein	-
H7R53_RS25265	24969..25244	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R53_RS25270	25244..25528	-	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
H7R53_RS25275	26436..27467	-	1032	WP_005082530.1	replication initiation protein	-
H7R53_RS25280	28304..28810	+	507	WP_005082529.1	transcription termination factor NusG	-
H7R53_RS25285	28950..29612	+	663	WP_005082527.1	hypothetical protein	-
H7R53_RS25290	29879..30022	+	144	WP_000176500.1	hypothetical protein	-
H7R53_RS25295	30103..30345	+	243	WP_001271734.1	PilI type IV pilus biogenesis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qacE / dfrA5	-	1..80029	80029
	-	flank	IS/Tn	sul1 / qacE / dfrA5	-	16062..20008	3946

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T34455 WP_000421257.1 NZ_AP022090:c25244-24969 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T34455 NZ_AP022090:c25244-24969 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT34455 WP_001178087.1 NZ_AP022090:c25528-25244 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT34455 NZ_AP022090:c25528-25244 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCTCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: U9Y625