Detailed information of TA system TA034454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 39926..40724
• Replicon: plasmid pWP4-S18-ESBL-07_2
• Accession: NZ_AP022089
• Organism: Escherichia coli strain WP4-S18-ESBL-07

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: H7R53_RS24665
• Protein ID: WP_097292383.1
• Coordinates: 39926..40447 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A0V9R6Q7
• Locus tag: H7R53_RS24670
• Protein ID: WP_001351987.1
• Coordinates: 40455..40724 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R53_RS24645	35607..38273	+	2667	WP_182920561.1	tail fiber protein	-
H7R53_RS24650	38476..38799	+	324	WP_000064175.1	hypothetical protein	-
H7R53_RS24655	38813..39505	+	693	WP_012640731.1	hypothetical protein	-
H7R53_RS24660	39507..39758	+	252	WP_001404395.1	hypothetical protein	-
H7R53_RS24665	39926..40447	-	522	WP_097292383.1	GNAT family N-acetyltransferase	Toxin
H7R53_RS24670	40455..40724	-	270	WP_001351987.1	DUF1778 domain-containing protein	Antitoxin
H7R53_RS24675	41043..41711	+	669	WP_000161228.1	AAA family ATPase	-
H7R53_RS24680	41717..42070	+	354	WP_160394000.1	hypothetical protein	-
H7R53_RS24685	42122..42874	+	753	WP_021520118.1	hypothetical protein	-
H7R53_RS24690	42867..43289	+	423	WP_021520119.1	hypothetical protein	-
H7R53_RS24695	43470..44210	+	741	WP_021520120.1	hypothetical protein	-
H7R53_RS24700	44254..45594	+	1341	WP_000137335.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110167	110167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19417.27 Da
• Isoelectric Point: 8.9805

>T34454 WP_097292383.1 NZ_AP022089:c40447-39926 [Escherichia coli]
VDNIKIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEGRPKVLGYYTLSGSCFEKESLPSRSQQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T34454 NZ_AP022089:c40447-39926 [Escherichia coli]
GTGGATAATATAAAGATAGAAATTTTCTCTGGAGAGAAAGATTATGATTTGAATGGTTTTGATTGCGGTGAAGAATCACT
AAATGCCTTTTTAACCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGCGCTTATGTGCTTTGCACGAAAGAAG
GCAGACCAAAAGTGTTAGGGTATTACACTTTGTCAGGTAGCTGCTTTGAAAAGGAGTCCTTACCTTCAAGAAGCCAGCAA
AAGAAGGTACCTTATCGGAATGTTCCAAGTGTTACTTTGGGTAGGCTGGCTTTGGACAAGTCCCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGGCTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10082.54 Da
• Isoelectric Point: 7.0155

>AT34454 WP_001351987.1 NZ_AP022089:c40724-40455 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Nucleotide

• Length: 270 bp

>AT34454 NZ_AP022089:c40724-40455 [Escherichia coli]
ATGTCAGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTGAAAGTTATAGAGCAACACCGTAGAC
TAGTTCTGAATGAAGAATCCTGGAATCTGGTTATGGACGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCTGATCGTCTGAAAAGCATGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0V9R6Q7