Detailed information of TA system TA034447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 26961..27496
• Replicon: plasmid pWP4-S18-ESBL-07_1
• Accession: NZ_AP022088
• Organism: Escherichia coli strain WP4-S18-ESBL-07

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T1PX77
• Locus tag: H7R53_RS23620
• Protein ID: WP_001523468.1
• Coordinates: 26961..27248 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T1PXV2
• Locus tag: H7R53_RS23625
• Protein ID: WP_001139206.1
• Coordinates: 27245..27496 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R53_RS23590	22244..22434	-	191	Protein_16	transposase	-
H7R53_RS23595	22434..22502	+	69	Protein_17	IS200/IS605 family transposase	-
H7R53_RS23600	22486..23184	-	699	WP_085326172.1	helix-turn-helix domain-containing protein	-
H7R53_RS23605	24186..24773	+	588	WP_072645656.1	proQ/FINO family protein	-
H7R53_RS23610	25053..25640	-	588	WP_064550130.1	colanic acid biosynthesis acetyltransferase	-
H7R53_RS23615	26406..26951	-	546	WP_170852536.1	hypothetical protein	-
H7R53_RS23620	26961..27248	-	288	WP_001523468.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R53_RS23625	27245..27496	-	252	WP_001139206.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H7R53_RS23630	27583..27750	-	168	WP_072645565.1	hypothetical protein	-
H7R53_RS23635	28450..29472	-	1023	WP_085008816.1	replication initiation protein	-
H7R53_RS23640	30433..30680	+	248	Protein_26	hypothetical protein	-
H7R53_RS23645	31326..31859	+	534	WP_001372168.1	transcription termination factor NusG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..183772	183772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11142.18 Da
• Isoelectric Point: 10.6741

>T34447 WP_001523468.1 NZ_AP022088:c27248-26961 [Escherichia coli]
MTYTVKFRDDALREWLRLDKAIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Nucleotide

• Length: 288 bp

>T34447 NZ_AP022088:c27248-26961 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTCAGACTGGATAAGGCCATTCAGCAGCAGTTCGC
AAAAAAACTAAAAAAATGCAGTGAAAATCCTCATATAGCCTCTGCAAAACTACGTGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9171.39 Da
• Isoelectric Point: 4.0927

>AT34447 WP_001139206.1 NZ_AP022088:c27496-27245 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT34447 NZ_AP022088:c27496-27245 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGTTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCAGCTGAGCTCTATGAAAGGATGC
TTGATGCCCTGGATGACCAGGAGCTGGTGAAACTGGTTACTGAGCGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: T1PX77

Antitoxin

• AlphaFold DB: T1PXV2