Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4167292..4167842 Replicon chromosome
Accession NZ_AP022087
Organism Escherichia coli strain WP4-S18-ESBL-07

Toxin (Protein)


Gene name ccdB Uniprot ID A0A244BU83
Locus tag H7R53_RS19705 Protein ID WP_001160962.1
Coordinates 4167292..4167606 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag H7R53_RS19710 Protein ID WP_000125566.1
Coordinates 4167609..4167842 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R53_RS19680 4162967..4164253 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
H7R53_RS19685 4164253..4165242 + 990 WP_000241249.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
H7R53_RS19690 4165239..4166060 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
H7R53_RS19695 4166063..4166440 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
H7R53_RS19700 4166447..4167295 + 849 WP_021556397.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
H7R53_RS19705 4167292..4167606 - 315 WP_001160962.1 CcdB family protein Toxin
H7R53_RS19710 4167609..4167842 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
H7R53_RS19715 4167928..4168407 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
H7R53_RS19720 4168599..4170461 - 1863 WP_000377114.1 glutathione-regulated potassium-efflux system protein KefC -
H7R53_RS19725 4170454..4170984 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
H7R53_RS19730 4171092..4172423 - 1332 WP_001183198.1 MFS transporter -
H7R53_RS19735 4172482..4172769 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.79 Da        Isoelectric Point: 9.0851

>T34439 WP_001160962.1 NZ_AP022087:c4167606-4167292 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFIRIRPPERLNTILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T34439 NZ_AP022087:c4167606-4167292 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCTCCAGAACGGCTTAACA
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGGGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT34439 WP_000125566.1 NZ_AP022087:c4167842-4167609 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT34439 NZ_AP022087:c4167842-4167609 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A244BU83

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References