Detailed information of TA system TA034436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2612679..2613317
• Replicon: chromosome
• Accession: NZ_AP022087
• Organism: Escherichia coli strain WP4-S18-ESBL-07

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: H7R53_RS12385
• Protein ID: WP_000813795.1
• Coordinates: 2613141..2613317 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H7R53_RS12380
• Protein ID: WP_001270286.1
• Coordinates: 2612679..2613095 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R53_RS12360	2607831..2608772	-	942	WP_024216494.1	ABC transporter permease	-
H7R53_RS12365	2608773..2609786	-	1014	WP_024042137.1	ABC transporter ATP-binding protein	-
H7R53_RS12370	2609804..2610949	-	1146	WP_024042138.1	ABC transporter substrate-binding protein	-
H7R53_RS12375	2611194..2612600	-	1407	WP_024042139.1	PLP-dependent aminotransferase family protein	-
H7R53_RS12380	2612679..2613095	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H7R53_RS12385	2613141..2613317	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H7R53_RS12390	2613539..2613769	+	231	WP_000494244.1	YncJ family protein	-
H7R53_RS12395	2613861..2615822	-	1962	WP_024216495.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H7R53_RS12400	2615895..2616431	-	537	WP_024044350.1	DNA-binding transcriptional regulator SutR	-
H7R53_RS12405	2616484..2617695	+	1212	WP_071604163.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T34436 WP_000813795.1 NZ_AP022087:c2613317-2613141 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T34436 NZ_AP022087:c2613317-2613141 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT34436 WP_001270286.1 NZ_AP022087:c2613095-2612679 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT34436 NZ_AP022087:c2613095-2612679 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCCTTACTCACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1